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A microneedle vaccine printer for thermostable COVID-19 mRNA vaccines.
Vander Straeten, Aurélien; Sarmadi, Morteza; Daristotle, John L; Kanelli, Maria; Tostanoski, Lisa H; Collins, Joe; Pardeshi, Apurva; Han, Jooli; Varshney, Dhruv; Eshaghi, Behnaz; Garcia, Johnny; Forster, Timothy A; Li, Gary; Menon, Nandita; Pyon, Sydney L; Zhang, Linzixuan; Jacob-Dolan, Catherine; Powers, Olivia C; Hall, Kevin; Alsaiari, Shahad K; Wolf, Morris; Tibbitt, Mark W; Farra, Robert; Barouch, Dan H; Langer, Robert; Jaklenec, Ana.
  • Vander Straeten A; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Sarmadi M; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Daristotle JL; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Kanelli M; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Tostanoski LH; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Collins J; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Pardeshi A; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Han J; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Varshney D; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Eshaghi B; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Garcia J; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Forster TA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Li G; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Menon N; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Pyon SL; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Zhang L; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Jacob-Dolan C; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Powers OC; Harvard Medical School, Boston, MA, USA.
  • Hall K; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Alsaiari SK; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Wolf M; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Tibbitt MW; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Farra R; Macromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, Zurich, Switzerland.
  • Barouch DH; Macromolecular Engineering Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, Zurich, Switzerland.
  • Jaklenec A; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Nat Biotechnol ; 2023 Apr 24.
Article in English | MEDLINE | ID: covidwho-2305153
ABSTRACT
Decentralized manufacture of thermostable mRNA vaccines in a microneedle patch (MNP) format could enhance vaccine access in low-resource communities by eliminating the need for a cold chain and trained healthcare personnel. Here we describe an automated process for printing MNP Coronavirus Disease 2019 (COVID-19) mRNA vaccines in a standalone device. The vaccine ink is composed of lipid nanoparticles loaded with mRNA and a dissolvable polymer blend that was optimized for high bioactivity by screening formulations in vitro. We demonstrate that the resulting MNPs are shelf stable for at least 6 months at room temperature when assessed using a model mRNA construct. Vaccine loading efficiency and microneedle dissolution suggest that efficacious, microgram-scale doses of mRNA encapsulated in lipid nanoparticles could be delivered with a single patch. Immunizations in mice using manually produced MNPs with mRNA encoding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein receptor-binding domain stimulate long-term immune responses similar to those of intramuscular administration.
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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Journal subject: Biotechnology Year: 2023 Document Type: Article Affiliation country: S41587-023-01774-z

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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Journal subject: Biotechnology Year: 2023 Document Type: Article Affiliation country: S41587-023-01774-z