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Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro.
Wang, Xue; Liu, Yi; Li, Kaiyuan; Yang, Ming; Wang, Qingtao; Hao, Zhihui.
  • Wang X; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • Liu Y; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • Li K; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • Yang M; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
  • Wang Q; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
  • Hao Z; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Int J Mol Sci ; 23(23)2022 Nov 22.
Article in English | MEDLINE | ID: covidwho-2305518
ABSTRACT
PEDV represents an ancient Coronavirus still causing huge economic losses to the porcine breeding industry. Resveratrol has excellent antiviral effects. Triacetyl resveratrol (TCRV), a novel natural derivative of resveratrol, has been recently discovered, and its pharmacological effects need to be explored further. This paper aims to explore the relationship between PEDV and TCRV, which offers a novel strategy in the research of antivirals. In our study, Vero cells and IPEC-J2 cells were used as an in vitro model. First, we proved that TCRV had an obvious anti-PEDV effect and a strong inhibitory effect at different time points. Then, we explored the mechanism of inhibition of PEDV infection by TCRV. Our results showed that TCRV could induce the early apoptosis of PEDV-infected cells, in contrast to PEDV-induced apoptosis. Moreover, we observed that TCRV could promote the expression and activation of apoptosis-related proteins and release mitochondrial cytochrome C into cytoplasm. Based on these results, we hypothesized that TCRV induced the early apoptosis of PEDV-infected cells and inhibited PEDV infection by activating the mitochondria-related caspase pathway. Furthermore, we used the inhibitors Z-DEVD-FMK and Pifithrin-α (PFT-α) to support our hypothesis. In conclusion, the TCRV-activated caspase pathway triggered early apoptosis of PEDV-infected cells, thereby inhibiting PEDV infections.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine Diseases / Porcine epidemic diarrhea virus Limits: Animals Language: English Year: 2022 Document Type: Article Affiliation country: Ijms232314499

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine Diseases / Porcine epidemic diarrhea virus Limits: Animals Language: English Year: 2022 Document Type: Article Affiliation country: Ijms232314499