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Rapid and Accurate Detection of SARS-CoV-2 Using an iPad-Controlled, High-Throughput, Portable, and Multiplex Hive-Chip Platform (HiCube).
Zhu, Yuanshou; Odiwuor, Nelson; Sha, Zigan; Chen, Yanjing; Shao, Ning; Wu, Xudong; Chen, Jianwei; Li, Yang; Guo, Shujuan; Shi, Dawei; Liu, Peng; Zhang, Yan; Wei, Hongping; Tao, Sheng-Ce.
  • Zhu Y; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
  • Odiwuor N; School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Sha Z; CAS Key Laboratory of Special Pathogens and Biosafety, Centre for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
  • Chen Y; University of Chinese Academy of Science, Beijing 100049, China.
  • Shao N; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
  • Wu X; Sports & Medicine Integrative Innovation Center (SMIC), Capital University of Physical Education and Sports, Beijing 100191, China.
  • Chen J; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
  • Li Y; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Guo S; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
  • Shi D; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
  • Liu P; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
  • Zhang Y; National Institutes for Food and Drug Control, Beijing 102629, China.
  • Wei H; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Tao SC; Sports & Medicine Integrative Innovation Center (SMIC), Capital University of Physical Education and Sports, Beijing 100191, China.
ACS Sens ; 8(5): 1960-1970, 2023 05 26.
Article in English | MEDLINE | ID: covidwho-2306620
ABSTRACT
Rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the most effective measures to control the coronavirus disease 2019 (COVID-19) pandemic. However, there is still lack of an ideal detection platform capable of high sample throughput, portability, and multiplicity. Herein, by combining Hive-Chip (capillary microarray) and reverse transcriptional loop-mediated isothermal amplification (RT-LAMP), we developed an iPad-controlled, high-throughput (48 samples at one run), portable (smaller than a backpack), multiplex (monitoring 8 gene fragments in one reaction), and real-time detection platform for SARS-CoV-2 detection. This platform is composed of a portable Hive-Chip device (HiCube; 32.7 × 29.7 × 20 cm, 5 kg), custom-designed software, and optimized Hive-Chips. RT-LAMP primers targeting seven SARS-CoV-2 genes (S, E, M, N, ORF1ab, ORF3a, and ORF7a) and one positive control (human RNase P) were designed and prefixed in the Hive-Chip. On-chip RT-LAMP showed that the limit of detection (LOD) of SARS-CoV-2 synthetic RNAs is 1 copy/µL, and there is no cross-reaction among different target genes. The platform was validated by 100 clinical samples of SARS-CoV-2, and the results were highly consistent with those of the traditional real-time PCR assay. In addition, on-chip detection of 6 other respiratory pathogens showed no cross-reactivity. Overall, our platform has great potential for fast, accurate, and on-site detection of SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: ACS Sens Year: 2023 Document Type: Article Affiliation country: Acssensors.3c00031

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: ACS Sens Year: 2023 Document Type: Article Affiliation country: Acssensors.3c00031