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Accurate and ultrasensitive detection for PEDV based on photoelectrochemical sensing coupling loop-mediated isothermal amplification.
Hong, Honghong; Yuan, Ruishuang; Ma, Hanyu; Xiao, Liting; Li, Bin; Wang, Kun.
  • Hong H; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
  • Yuan R; School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
  • Ma H; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
  • Xiao L; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
  • Li B; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
  • Wang K; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China; School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, OE, School of Chemistry and Molecula
Talanta ; 258: 124476, 2023 Jun 01.
Article in English | MEDLINE | ID: covidwho-2308939
ABSTRACT
Porcine epidemic diarrhea (PED) is a serious disease requiring a simple and accurate detection method. Accordingly, this study developed a novel, ultrasensitive photoelectrochemical (PEC) sensing platform using the loop-mediated isothermal amplification (LAMP) technique (LAMP-PEC). An amino (-NH2)-modified LAMP product is obtained by amplification of the PED virus gene with specially designed primers. The generated NH2-modified LAMP product is assembled on the surface of an electrode by forming imine linkages between aldehyde and amino groups based on the Schiff base reaction. A stable photocurrent is provided by a CdIn2S4 photoactive material, which possesses high photoelectric conversion efficiency. Amplified DNA assembled on the electrode surface increases steric hindrance and hinders electrons from moving from the electrode to electron acceptors, which decreases the photocurrent. This strategy can detect PEDV with a low detection limit of 0.3 fg µL-1 and a wide linear range of 1 × 10-3-1 × 102 pg/µL. The sensing platform has excellent specificity and sensitivity and can be used for the quantitative detection of many other pathogens with the assistance of LAMP.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: DNA / Nucleic Acid Amplification Techniques Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Animals Language: English Journal: Talanta Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: DNA / Nucleic Acid Amplification Techniques Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Animals Language: English Journal: Talanta Year: 2023 Document Type: Article