SARS-CoV-2 Nsp8 induces mitophagy by damaging mitochondria.
Virol Sin
; 2023 May 06.
Article
in English
| MEDLINE | ID: covidwho-2311879
ABSTRACT
Autophagy plays an important role in the interaction between viruses and host cells. SARS-CoV-2 infection can disrupt the autophagy process in target cells. However, the precise molecular mechanism is still unknown. In this study, we discovered that the Nsp8 of SARS-CoV-2 could cause an increasing accumulation of autophagosomes by preventing the fusion of autophagosomes and lysosomes. From further investigation, we found that Nsp8 was present on mitochondria and can damage mitochondria to initiate mitophagy. The results of experiments with immunofluorescence revealed that Nsp8 induced incomplete mitophagy. Moreover, both domains of Nsp8 orchestrated their function during Nsp8-induced mitophagy, in which the N-terminal domain colocalized with mitochondria and the C-terminal domain induced auto/mitophagy. This novel finding expands our understanding of the function of Nsp8 in promoting mitochondrial damage and inducing incomplete mitophagy, which helps us to understand the etiology of COVID-19 as well as open up new pathways for creating SARS-CoV-2 treatment methods.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Type of study:
Etiology study
Language:
English
Journal subject:
Virology
Year:
2023
Document Type:
Article
Affiliation country:
J.virs.2023.05.003
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