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Design and validation of Dolosigranulum pigrum specific PCR primers using the bacterial core genome.
Aziz, Maliha; Palmer, Amber; Iversen, Søren; Salazar, Juan E; Pham, Tony; Roach, Kelsey; Becker, Karsten; Kaspar, Ursula; Price, Lance B; Baig, Sharmin; Stegger, Marc; Andersen, Paal Skytt; Liu, Cindy M.
  • Aziz M; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
  • Palmer A; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
  • Iversen S; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
  • Salazar JE; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
  • Pham T; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
  • Roach K; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
  • Becker K; Friedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
  • Kaspar U; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
  • Price LB; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
  • Baig S; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
  • Stegger M; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
  • Andersen PS; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
  • Liu CM; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA. cindyliu@gwu.edu.
Sci Rep ; 13(1): 6110, 2023 04 14.
Article in English | MEDLINE | ID: covidwho-2312766
ABSTRACT
Dolosigranulum pigrum-a lactic acid bacterium that is increasingly recognized as an important member of the nasal microbiome. Currently, there are limited rapid and low-cost options for confirming D. pigrum isolates and detecting D. pigrum in clinical specimens. Here we describe the design and validation of a novel PCR assay targeting D. pigrum that is both sensitive and specific. We designed a PCR assay targeting murJ, a single-copy core species gene identified through the analysis of 21 D. pigrum whole genome sequences. The assay achieved 100% sensitivity and 100% specificity against D. pigrum and diverse bacterial isolates and an overall 91.1% sensitivity and 100% specificity using nasal swabs, detecting D. pigrum at a threshold of 1.0 × 104 D. pigrum 16S rRNA gene copies per swab. This assay adds a reliable and rapid D. pigrum detection tool to the microbiome researcher toolkit investigating the role of generalist and specialist bacteria in the nasal environment.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Gram-Positive Bacterial Infections / Gram-Positive Cocci Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2023 Document Type: Article Affiliation country: S41598-023-32709-y

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Gram-Positive Bacterial Infections / Gram-Positive Cocci Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2023 Document Type: Article Affiliation country: S41598-023-32709-y