Therapeutic Effect of Gegentang Granules on Mouse Model with hCoV-229E Pneumonia and Hanshi Yidu Xifei Syndrome.
Chinese Journal of Experimental Traditional Medical Formulae
; 28(19):34-41, 2022.
Article
in Chinese
| EMBASE | ID: covidwho-2314803
ABSTRACT
Objective:
To determine the therapeutic effect of Gegentang granules on a disease-syndrome mouse model combining human coronavirus 229EhCoV-229Epneumonia with Hanshi Yidu Xifei syndrome in vivo. Method(s) Mice were randomly divided into normal group,infection group,cold-dampness group,model group,chloroquine phosphate group0.18 g.kg-1,interferon-alpha2bIFN-alpha2bgroup1.83x106 U.kg-1, Gegentang granules high-dose and low-dose groups6.6,3.3 g.kg-1with 10 mice in each group. Cold-dampness environment and hCoV-229E infection were used for modeling,and the general status and lung index of mice in each group were observed. The viral load in lung tissue was detected by real-time fluorescent quantitative polymerase chain reactionReal-time PCR,the pathological changes in lung tissue were evaluated by hematoxylin-eosinHEstaining,the levels of serum gastrointestinal hormones and inflammatory factors in lung tissue were detected by enzyme-linked immunosorbent assayELISA,and the percentage of peripheral blood lymphocytes was detected by flow cytometry. Result(s)Comparing with model group,Gegentang granules could significantly alleviate the physical signs of Hanshi Yidu Xifei syndrome,including listlessness,weakness of limbs,sticky stool,etc. Comparing with model group,Gegentang granules high-dose group significantly reduced lung index,histopathological score of interstitial lung and bronchus,and the level of serum motilinP< 0.05,P<0.01,two doses of Gegentang granules could significantly increase the level of serum gastrinP< 0.05,P<0.01,the percentage of CD4+ ,CD8+ T lymphocytes in peripheral bloodP<0.05,P<0.01,and the level of tumor necrosis factor-alphaTNF-alphain lung tissue was significantly decreasedP<0.01,and the level of interleukin-6IL-6showed decreasing tendency. Conclusion(s) Gegentang granules has therapeutic effect on model mice. It can improve the appearance and behavior characterization,regulate the level of gastrointestinal hormones,decrease lung index and histopathological score,and possibly play an immunomodulatory role by inhibiting the expression of inflammatory cytokines in lung tissue and restoring the percentage of peripheral blood lymphocytes.Copyright © 2022, China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica. All rights reserved.
Gegentang granules; Hanshi Yidu Xifei syndrome; human coronavirus 229EhCoV-229E; immune regulation; interleukinIL; tumor necrosis factorTNF; viral pneumonia; animal experiment; animal model; animal tissue; article; CD4+ T lymphocyte; CD8+ T lymphocyte; controlled study; coronavirus disease 2019/dt [Drug Therapy]; enzyme linked immunosorbent assay; flow cytometry; fluorescence quantitative polymerase chain reaction; histopathology; Human coronavirus 229E; immunoregulation; limb weakness; listlessness; lung disease/dt [Drug Therapy]; mouse; nonhuman; protein expression; therapy effect; virus load; alpha2b interferon; chloroquine; gastrin/ec [Endogenous Compound]; gastrointestinal hormone/ec [Endogenous Compound]; interleukin 6/ec [Endogenous Compound]; kakkon-to/dt [Drug Therapy]; motilin/ec [Endogenous Compound]; tumor necrosis factor/ec [Endogenous Compound]; Hanshi Yidu Xifei syndrome/dt [Drug Therapy]
Full text:
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Collection:
Databases of international organizations
Database:
EMBASE
Type of study:
Experimental Studies
Language:
Chinese
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2022
Document Type:
Article
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