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Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications.
Kara, Nilufer; Ayoub, Nooraldeen; Ilgu, Huseyin; Fotiadis, Dimitrios; Ilgu, Muslum.
  • Kara N; Department of Biological Sciences, Middle East Technical University, Ankara 06800, Turkey.
  • Ayoub N; Department of Biological Sciences, Middle East Technical University, Ankara 06800, Turkey.
  • Ilgu H; Institute of Biochemistry and Molecular Medicine, University of Bern, CH-3012 Bern, Switzerland.
  • Fotiadis D; Institute of Biochemistry and Molecular Medicine, University of Bern, CH-3012 Bern, Switzerland.
  • Ilgu M; Institute of Biochemistry and Molecular Medicine, University of Bern, CH-3012 Bern, Switzerland.
Molecules ; 28(9)2023 Apr 26.
Article in English | MEDLINE | ID: covidwho-2315908
ABSTRACT
Many biological processes (physiological or pathological) are relevant to membrane proteins (MPs), which account for almost 30% of the total of human proteins. As such, MPs can serve as predictive molecular biomarkers for disease diagnosis and prognosis. Indeed, cell surface MPs are an important class of attractive targets of the currently prescribed therapeutic drugs and diagnostic molecules used in disease detection. The oligonucleotides known as aptamers can be selected against a particular target with high affinity and selectivity by iterative rounds of in vitro library evolution, known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). As an alternative to antibodies, aptamers offer unique features like thermal stability, low-cost, reuse, ease of chemical modification, and compatibility with various detection techniques. Particularly, immobilized-aptamer sensing platforms have been under investigation for diagnostics and have demonstrated significant value compared to other analytical techniques. These "aptasensors" can be classified into several types based on their working principle, which are commonly electrochemical, optical, or mass-sensitive. In this review, we review the studies on aptamer-based MP-sensing technologies for diagnostic applications and have included new methodological variations undertaken in recent years.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Aptamers, Nucleotide Type of study: Diagnostic study / Prognostic study / Systematic review/Meta Analysis Limits: Humans Language: English Journal subject: Biology Year: 2023 Document Type: Article Affiliation country: Molecules28093728

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Aptamers, Nucleotide Type of study: Diagnostic study / Prognostic study / Systematic review/Meta Analysis Limits: Humans Language: English Journal subject: Biology Year: 2023 Document Type: Article Affiliation country: Molecules28093728