Purification and characterisation of heparin-like sulfated polysaccharides with potent anti-SARS-CoV-2 activity from snail mucus of Achatina fulica.
Carbohydr Res
; 529: 108832, 2023 Jul.
Article
in English
| MEDLINE | ID: covidwho-2316161
ABSTRACT
Heparin-like sulfated polysaccharide, acharan sulfate, was purified from the mucus of an African giant snail with unique sulfated glycosaminoglycans (GAGs). This study reported on finding novel and safe heparin resources from Achatina fulica for further use as well as easy isolation and purification of the active fraction from the initial raw material. Its structure was characterised by a strong-anion exchange combined with high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy. The results indicated that the potential acharan sulfate fraction is a glycosaminoglycan composed of several repeating disaccharide units, namely, of â4)-α-IdoA(2S)(1â4)-α-GlcNAc/GlcNAc(6S)/GlcNSO3(6S)(1â, and hence, presents heterogeneity regarding negative net charge density. Furthermore, the heparinase digests inhibit the binding of SARS-CoV-2 spike protein to the ACE2 receptor. In summary, the acharan sulfate presented in this work has shown its great potential for application in the preparation of sulfated polysaccharides as an alternative to heparin with important biological activity.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Heparin
/
COVID-19
Limits:
Animals
/
Humans
Language:
English
Journal:
Carbohydr Res
Year:
2023
Document Type:
Article
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