Mini-thermal platform integrated with microfluidic device with on-site detection for real-time DNA amplification.
Biotechniques
; 74(4): 158-171, 2023 04.
Article
in English
| MEDLINE | ID: covidwho-2316281
ABSTRACT
The recent cases of COVID-19 have brought the prospect of and requirement for point-of-care diagnostic devices into the limelight. Despite all the advances in point-of-care devices, there is still a huge requirement for a rapid, accurate, easy-to-use, low-cost, field-deployable and miniaturized PCR assay device to amplify and detect genetic material. This work aims to develop an Internet-of-Things automated, integrated, miniaturized and cost-effective microfluidic continuous flow-based PCR device capable of on-site detection. As a proof of application, the 594-bp GAPDH gene was successfully amplified and detected on a single system. The presented mini thermal platform with an integrated microfluidic device has the potential to be used for the detection of several infectious diseases.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
COVID-19
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Biotechniques
Year:
2023
Document Type:
Article
Affiliation country:
Btn-2022-0091
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