Isolation, identification, and S1 gene sequencing of two strains of Avian Infectious bronchitis virus
Fujian Journal of Agricultural Sciences
; 37(11):1388-1393, 2022.
Article
in Chinese
| CAB Abstracts | ID: covidwho-2316627
ABSTRACT
Objective:
Epidemiology and genetic variations of the infectious bronchitis virus(IBV) in Fujian province were studied.Method:
Two strains of virus isolated from the diseased chickens in Fujian in 2021 were identified by chicken embryo pathogenicity test, electron microscope observation, and RT-PCR. S1 genes of the isolates were cloned, sequenced, and analyzed using biological software.Result:
The two IBV strains were code named FJ-NP01 and FJ-FZ01. The full length of S1 of FJ-NP01 was 1 629 nt encoding 543 amino acids, and that of FJ-FZ01, 1 620 nt encoding 540 amino acids. The S1 gene cleavage site of FJ-FZ01 was HRRRR, same as all reference strains of genotype I branch;while that of FJ-NP01 HRRKR differed from the reported site of IBV isolated from genotype IV but same as that of TC07-2 reference strain of genotype VI. The homology of nucleotide and amino acid between the two isolates was 83.2% and 79.6%, respectively, but merely 75.7%-76.3%and 77.1%-83.5% with the Mass-type conventional vaccines H120 and H52, respectively. Further analysis showed that FJ-NP01was from a recombination event between CK CH GD LZ12-4 and L-1148, the homology of nucleotide acid between 1438-1506 nt of FJ-NP01 with CK CH GD LZ12-4 was 97%, and 95.9% between the other nucleotide acid of S1 gene with L-1148.Conclusion:
It appeared that the IBV epidemic experienced in the province was complex in nature and that the existing Mass vaccines would not provide sufficient immune protection to deter the spread.
Prion; Viral; Bacterial and Fungal Pathogens of Animals [LL821]; Non-communicable Diseases and Injuries of Animals [LL860]; Diagnosis of Animal Diseases [LL886]; Molecular Biology and Molecular Genetics [ZZ360]; Taxonomy and Evolution [ZZ380]; Genetics and Molecular Biology of Microorganisms [ZZ395]; avian infectious bronchitis; avian leukosis; chick embryos; disease prevalence; DNA cloning; electron microscopy; epidemiology; genes; genetic variation; genotypes; neoplasms; nucleotide sequences; pathogenicity; phylogenetics; recombination; reverse transcriptase PCR; strains; poultry; Avian leukosis virus; fowls; Infectious bronchitis virus; China; Fujian; Alpharetrovirus; Orthoretrovirinae; Retroviridae; RNA Reverse Transcribing Viruses; viruses; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; Gallus gallus; Gallus; Phasianidae; Galliformes; birds; vertebrates; Chordata; animals; eukaryotes; Eastern China; Avian coronavirus; Gammacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; IBV infection; People's Republic of China; chickens; Fukien; genetic variability; genotypic variability; genotypic variation; cancers; DNA sequences; genetic recombination; reverse transcriptase polymerase chain reaction; RT-PCR; domesticated birds
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
Chinese
Journal:
Fujian Journal of Agricultural Sciences
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS