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Current genetic strategies to investigate gene functions in Trichoderma reesei.
Ma, Chixiang; Liu, Jialong; Tang, Jiaxin; Sun, Yuanlu; Jiang, Xiaojie; Zhang, Tongtong; Feng, Yan; Liu, Qinghua; Wang, Lei.
  • Ma C; China Medical University-The Queen's University of Belfast Joint College, Shenyang, Liaoning, 110122, China.
  • Liu J; College of Basic Medical Sciences, Shanxi Medical University, Taiyuan, 030001, Shanxi, China.
  • Tang J; College of Basic Medical Sciences, Shanxi Medical University, Taiyuan, 030001, Shanxi, China.
  • Sun Y; China Medical University-The Queen's University of Belfast Joint College, Shenyang, Liaoning, 110122, China.
  • Jiang X; China Medical University-The Queen's University of Belfast Joint College, Shenyang, Liaoning, 110122, China.
  • Zhang T; China Medical University-The Queen's University of Belfast Joint College, Shenyang, Liaoning, 110122, China.
  • Feng Y; College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China.
  • Liu Q; College of Basic Medical Sciences, Shanxi Medical University, Taiyuan, 030001, Shanxi, China.
  • Wang L; College of Basic Medical Sciences, Shanxi Medical University, Taiyuan, 030001, Shanxi, China. wanglei@sxmu.edu.cn.
Microb Cell Fact ; 22(1): 97, 2023 May 10.
Article in English | MEDLINE | ID: covidwho-2316790
ABSTRACT
The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina, Ascomycota) is a well-known lignocellulolytic enzymes-producing strain in industry. To increase the fermentation titer of lignocellulolytic enzymes, random mutagenesis and rational genetic engineering in T. reesei were carried out since it was initially found in the Solomon Islands during the Second World War. Especially the continuous exploration of the underlying regulatory network during (hemi)cellulase gene expression in the post-genome era provided various strategies to develop an efficient fungal cell factory for these enzymes' production. Meanwhile, T. reesei emerges competitiveness potential as a filamentous fungal chassis to produce proteins from other species (e.g., human albumin and interferon α-2b, SARS-CoV-2 N antigen) in virtue of the excellent expression and secretion system acquired during the studies about (hemi)cellulase production. However, all the achievements in high yield of (hemi)cellulases are impossible to finish without high-efficiency genetic strategies to analyze the proper functions of those genes involved in (hemi)cellulase gene expression or secretion. Here, we in detail summarize the current strategies employed to investigate gene functions in T. reesei. These strategies are supposed to be beneficial for extending the potential of T. reesei in prospective strain engineering.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cellulase / COVID-19 Type of study: Cohort study / Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Microb Cell Fact Journal subject: Biotechnology / Microbiology Year: 2023 Document Type: Article Affiliation country: S12934-023-02104-3

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cellulase / COVID-19 Type of study: Cohort study / Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Microb Cell Fact Journal subject: Biotechnology / Microbiology Year: 2023 Document Type: Article Affiliation country: S12934-023-02104-3