A New CURE in Molecular Biology: Testing a Novel Pyrrolidine Compound to Determine Mechanism of Action in an Upper-Division Molecular Biology Course
Journal of Biological Chemistry
; 299(3 Supplement):S283, 2023.
Article
in English
| EMBASE | ID: covidwho-2317379
ABSTRACT
The COVID-19 pandemic shut down forced introductory biology and chemistry laboratory courses online at DePauw University from March 2020-June 2021, leaving multiple classes of students without the opportunity to learn basic laboratory skills that are essential for the molecular biology laboratory. In an effort to provide students with both basic laboratory skills and advanced molecular biology skills, a new course-based undergraduate research experience (CURE) was developed for the 2022-23 academic year. In collaboration with Dr. Jeff Hansen in the Chemistry and Biochemistry department, novel compounds with potential anti-tumor properties were identified. The CURE in Molecular Biology was designed to have students use Saccharomyces cerevisiae as a model system to evaluate possible cellular pathways affected by the compound, including cytoskeleton and cell migration, nucleotide biosynthesis, glucose metabolism, apoptosis, and cell cycle regulation. Students learned techniques DNA isolation and PCR, transformation, RNA isolation, cDNA synthesis, qPCR, and Western Blotting, while contributing to an active research project. At the conclusion of the project, students were surveyed about their comfort with molecular techniques and data analysis.Copyright © 2023 The American Society for Biochemistry and Molecular Biology, Inc.
apoptosis; biochemistry; biosynthesis; cell cycle regulation; cell migration; comfort; conference abstract; controlled study; cytoskeleton; data analysis; DNA isolation; glucose metabolism; human; human experiment; molecular biology; neoplasm; nonhuman; polymerase chain reaction; RNA isolation; Saccharomyces cerevisiae; synthesis; Western blotting; complementary DNA; nucleotide
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Type of study:
Prognostic study
Language:
English
Journal:
Journal of Biological Chemistry
Year:
2023
Document Type:
Article
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