Your browser doesn't support javascript.
A novel anterior nasal swab to detect respiratory viruses: a prospective study of diagnostic accuracy.
Tosif, Shidan; Lee, Lai-Yang; Nguyen, Jill; Overmars, Isabella; Selman, Chris; Grobler, Anneke C; McMinn, Alissa; Waller, Gregory; McNab, Sarah; Jarvis, Tayla; Steer, Andrew; Babl, Franz E; Daley, Andrew; Crawford, Nigel W.
  • Tosif S; Department of General Medicine, The Royal Children's Hospital Melbourne, Melbourne, Australia. shidan.tosif@rch.org.au.
  • Lee LY; Infection and Immunity, Murdoch Children's Research Institute, 50 Flemington Rd, Melbourne, VIC, 3052, Australia. shidan.tosif@rch.org.au.
  • Nguyen J; Department of Paediatrics, The University of Melbourne, Melbourne, Australia. shidan.tosif@rch.org.au.
  • Overmars I; Department of Microbiology, The Royal Children's Hospital Melbourne, Melbourne, Australia.
  • Selman C; Infection and Immunity, Murdoch Children's Research Institute, 50 Flemington Rd, Melbourne, VIC, 3052, Australia.
  • Grobler AC; Infection and Immunity, Murdoch Children's Research Institute, 50 Flemington Rd, Melbourne, VIC, 3052, Australia.
  • McMinn A; Clinical Epidemiology & Biostatistics Unit, Murdoch Children's Research Institute, Melbourne, Australia.
  • Waller G; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
  • McNab S; Clinical Epidemiology & Biostatistics Unit, Murdoch Children's Research Institute, Melbourne, Australia.
  • Jarvis T; Infection and Immunity, Murdoch Children's Research Institute, 50 Flemington Rd, Melbourne, VIC, 3052, Australia.
  • Steer A; Department of Microbiology, The Royal Children's Hospital Melbourne, Melbourne, Australia.
  • Babl FE; Department of General Medicine, The Royal Children's Hospital Melbourne, Melbourne, Australia.
  • Daley A; Infection and Immunity, Murdoch Children's Research Institute, 50 Flemington Rd, Melbourne, VIC, 3052, Australia.
  • Crawford NW; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
BMC Pediatr ; 23(1): 201, 2023 04 28.
Article in English | MEDLINE | ID: covidwho-2326720
ABSTRACT
Detection of respiratory viruses requires testing of the upper respiratory tract to obtain specimens for analysis. However, nasal and throat swabs can cause discomfort and procedural anxiety in children. Respiratory sampling methods which are accurate and less invasive are needed. We aim to determine the positive and negative percentage agreement of a novel anterior nasal swab (ANS) compared with the combined throat and anterior nasal swab (CTN), the reference standard, for detection of respiratory viruses. Children 5 - 18 years of age presenting to a tertiary paediatric hospital with respiratory symptoms were tested with both swabs in randomised order. Respiratory samples were tested on a multiplex RT-PCR panel. Viral detections, RT-PCR cycle-threshold values and child/parent/clinician experience of the swab were recorded. There were 157 viral detections from 249 participant CTN swabs. In comparison with the CTN, the overall positive and negative percentage agreement of ANS for detection of respiratory viruses was 96.2% (95% CI, 91.8-98.3%) and 99.8% (95% CI, 99.6-99.9%), respectively. The ANS was "extremely comfortable", or only a "little uncomfortable" for 90% of children compared with 48% for CTN. 202 children (84%) rated the ANS as the preferred swab, and 208 (87%) indicated they would prefer ANS for future testing. The ANS required additional laboratory handling processes compared to the CTN. The ANS has high positive percentage agreement and is comparable to the current standard of care. The high acceptability from the less invasive ANS provides a more comfortable method for respiratory virus testing in children.Trial registrationClinicalTrials.gov ID NCT05043623.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Viruses Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Child / Humans Language: English Journal: BMC Pediatr Journal subject: Pediatrics Year: 2023 Document Type: Article Affiliation country: S12887-023-03976-5

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Viruses Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Child / Humans Language: English Journal: BMC Pediatr Journal subject: Pediatrics Year: 2023 Document Type: Article Affiliation country: S12887-023-03976-5