Your browser doesn't support javascript.
A neutralizing bispecific single-chain antibody against SARS-CoV-2 Omicron variant produced based on CR3022.
Yu, Kaikai; Liu, Bin; Yu, Haotian; Sun, Chengbiao; Wang, Xuefeng; Li, Guorui; Dong, Mingxin; Wang, Yan; Zhang, Jianxu; Xu, Na; Liu, Wensen.
  • Yu K; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
  • Liu B; Academic Affairs Office, Jilin Medical University, Jilin, Jilin, China.
  • Yu H; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
  • Sun C; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
  • Wang X; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
  • Li G; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Dong M; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
  • Wang Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
  • Zhang J; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
  • Xu N; College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, China.
  • Liu W; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Science, Changchun, Jilin, China.
Front Cell Infect Microbiol ; 13: 1155293, 2023.
Article in English | MEDLINE | ID: covidwho-2327276
ABSTRACT

Introduction:

The constantly mutating SARS-CoV-2 has been infected an increasing number of people, hence the safe and efficacious treatment are urgently needed to combat the COVID-19 pandemic. Currently, neutralizing antibodies (Nabs), targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein are potentially effective therapeutics against COVID-19. As a new form of antibody, bispecific single chain antibodies (BscAbs) can be easily expressed in E. coli and exhibits broad-spectrum antiviral activity.

Methods:

In this study, we constructed two BscAbs 16-29, 16-3022 and three single chain variable fragments (scFv) S1-16, S2-29 and S3022 as a comparison to explore their antiviral activity against SARS-CoV-2. The affinity of the five antibodies was characterized by ELISA and SPR and the neutralizing activity of them was analyzed using pseudovirus or authentic virus neutralization assay. Bioinformatics and competitive ELISA methods were used to identify different epitopes on RBD.

Results:

Our results revealed the potent neutralizing activity of two BscAbs 16-29 and 16-3022 against SARS-CoV-2 original strain and Omicron variant infection. In addition, we also found that SARS-CoV RBD-targeted scFv S3022 could play a synergistic role with other SARS-CoV-2 RBD-targeted antibodies to enhance neutralizing activity in the form of a BscAb or in cocktail therapies.

Discussion:

This innovative approach offers a promising avenue for the development of subsequent antibody therapies against SARSCoV-2. Combining the advantages of cocktails and single-molecule strategies, BscAb therapy has the potential to be developed as an effective immunotherapeutic for clinical use to mitigate the ongoing pandemic.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Single-Chain Antibodies / COVID-19 Type of study: Prognostic study Topics: Variants Limits: Humans Language: English Journal: Front Cell Infect Microbiol Year: 2023 Document Type: Article Affiliation country: Fcimb.2023.1155293

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Single-Chain Antibodies / COVID-19 Type of study: Prognostic study Topics: Variants Limits: Humans Language: English Journal: Front Cell Infect Microbiol Year: 2023 Document Type: Article Affiliation country: Fcimb.2023.1155293