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A serological assay to detect SARS-CoV-2 seroconversion in humans.
Amanat, Fatima; Stadlbauer, Daniel; Strohmeier, Shirin; Nguyen, Thi H O; Chromikova, Veronika; McMahon, Meagan; Jiang, Kaijun; Arunkumar, Guha Asthagiri; Jurczyszak, Denise; Polanco, Jose; Bermudez-Gonzalez, Maria; Kleiner, Giulio; Aydillo, Teresa; Miorin, Lisa; Fierer, Daniel S; Lugo, Luz Amarilis; Kojic, Erna Milunka; Stoever, Jonathan; Liu, Sean T H; Cunningham-Rundles, Charlotte; Felgner, Philip L; Moran, Thomas; García-Sastre, Adolfo; Caplivski, Daniel; Cheng, Allen C; Kedzierska, Katherine; Vapalahti, Olli; Hepojoki, Jussi M; Simon, Viviana; Krammer, Florian.
  • Amanat F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Stadlbauer D; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Strohmeier S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Nguyen THO; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Chromikova V; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
  • McMahon M; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia.
  • Jiang K; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Arunkumar GA; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Jurczyszak D; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Polanco J; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Bermudez-Gonzalez M; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Kleiner G; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Aydillo T; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Miorin L; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Fierer DS; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Lugo LA; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Kojic EM; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Stoever J; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Liu STH; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Cunningham-Rundles C; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Felgner PL; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Moran T; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • García-Sastre A; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Caplivski D; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Cheng AC; Division of Pulmonary, Critical Care, and Sleep Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Kedzierska K; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Vapalahti O; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Hepojoki JM; Department of Medicine, Mount Sinai Queens, Astoria, NY, USA.
  • Simon V; Division of Hospital Medicine, Mount Sinai Health System, New York, NY, USA.
  • Krammer F; Division of Clinical Immunology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Nat Med ; 26(7): 1033-1036, 2020 07.
Article in English | MEDLINE | ID: covidwho-244499
Preprint
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ABSTRACT
Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Seroconversion / Betacoronavirus Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Adult / Humans / Middle aged / Young adult Language: English Journal: Nat Med Journal subject: Molecular Biology / Medicine Year: 2020 Document Type: Article Affiliation country: S41591-020-0913-5

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Seroconversion / Betacoronavirus Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Adult / Humans / Middle aged / Young adult Language: English Journal: Nat Med Journal subject: Molecular Biology / Medicine Year: 2020 Document Type: Article Affiliation country: S41591-020-0913-5