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Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories.
LeBlanc, Jason J; Gubbay, Jonathan B; Li, Yan; Needle, Robert; Arneson, Sandra Radons; Marcino, Dionne; Charest, Hugues; Desnoyers, Guillaume; Dust, Kerry; Fattouh, Ramzi; Garceau, Richard; German, Gregory; Hatchette, Todd F; Kozak, Robert A; Krajden, Mel; Kuschak, Theodore; Lang, Amanda L S; Levett, Paul; Mazzulli, Tony; McDonald, Ryan; Mubareka, Samira; Prystajecky, Natalie; Rutherford, Candy; Smieja, Marek; Yu, Yang; Zahariadis, George; Zelyas, Nathan; Bastien, Nathalie.
  • LeBlanc JJ; Division of Microbiology, Department of Pathology and Laboratory Medicine, Nova Scotia Health Authority (NSHA), Room 404B, MacKenzie Building, 5788 University Avenue, Halifax, Nova Scotia B3H 1V8, Canada; Departments of Pathology, Medicine, and Microbiology and Immunology, Dalhousie University, Hali
  • Gubbay JB; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Public Health Ontario Laboratories, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
  • Li Y; National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, Manitoba, Canada.
  • Needle R; Public Health and Microbiology Laboratory, St. John's, Newfoundland, Canada.
  • Arneson SR; National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, Manitoba, Canada.
  • Marcino D; National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, Manitoba, Canada.
  • Charest H; Laboratoire De Santé Publique Du Québec/INSPQ, Sainte-Anne-de-Bellevue, Quebec, Canada.
  • Desnoyers G; Centre Hospitalier Universitaire Dr. Georges L. Dumont, Moncton, New-Brunswick, Canada.
  • Dust K; Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
  • Fattouh R; Department of Laboratory Medicine, St. Michael's Hospital, Toronto, Ontario, Canada.
  • Garceau R; Centre Hospitalier Universitaire Dr. Georges L. Dumont, Moncton, New-Brunswick, Canada.
  • German G; Queen Elizabeth Hospital, Charlottetown, Prince Edward Island, Canada.
  • Hatchette TF; Division of Microbiology, Department of Pathology and Laboratory Medicine, Nova Scotia Health Authority (NSHA), Room 404B, MacKenzie Building, 5788 University Avenue, Halifax, Nova Scotia B3H 1V8, Canada; Departments of Pathology, Medicine, and Microbiology and Immunology, Dalhousie University, Hali
  • Kozak RA; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Sunnybrook Research Institute, Toronto, Ontario, Canada.
  • Krajden M; Public Health Laboratory, British Columbia Centre for Disease Control (BCCDC), Vancouver, British Columbia, Canada.
  • Kuschak T; National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, Manitoba, Canada.
  • Lang ALS; Roy Romanow Provincial Laboratory (RRPL), Saskatchewan Health Authority (SHA), Regina, Saskatchewan, Canada.
  • Levett P; Public Health Laboratory, British Columbia Centre for Disease Control (BCCDC), Vancouver, British Columbia, Canada.
  • Mazzulli T; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada.
  • McDonald R; Roy Romanow Provincial Laboratory (RRPL), Saskatchewan Health Authority (SHA), Regina, Saskatchewan, Canada.
  • Mubareka S; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Sunnybrook Research Institute, Toronto, Ontario, Canada.
  • Prystajecky N; Public Health Laboratory, British Columbia Centre for Disease Control (BCCDC), Vancouver, British Columbia, Canada.
  • Rutherford C; St Joseph's Healthcare, Hamilton, Ontario, Canada.
  • Smieja M; St Joseph's Healthcare, Hamilton, Ontario, Canada.
  • Yu Y; Public Health and Microbiology Laboratory, St. John's, Newfoundland, Canada.
  • Zahariadis G; Public Health and Microbiology Laboratory, St. John's, Newfoundland, Canada.
  • Zelyas N; Division of Microbiology, Department of Pathology and Laboratory Medicine, Nova Scotia Health Authority (NSHA), Room 404B, MacKenzie Building, 5788 University Avenue, Halifax, Nova Scotia B3H 1V8, Canada; Provincial Laboratory for Public Health (ProvLab), Calgary, Alberta, Canada.
  • Bastien N; National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, Manitoba, Canada.
J Clin Virol ; 128: 104433, 2020 07.
Article in English | MEDLINE | ID: covidwho-245515
ABSTRACT
With emergence of pandemic COVID-19, rapid and accurate diagnostic testing is essential. This study compared laboratory-developed tests (LDTs) used for the detection of SARS-CoV-2 in Canadian hospital and public health laboratories, and some commercially available real-time RT-PCR assays. Overall, analytical sensitivities were equivalent between LDTs and most commercially available methods.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Pandemics / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Country/Region as subject: North America Language: English Journal: J Clin Virol Journal subject: Virology Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Pandemics / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Country/Region as subject: North America Language: English Journal: J Clin Virol Journal subject: Virology Year: 2020 Document Type: Article