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Positive detection of SARS-CoV-2 combined HSV1 and HHV6B virus nucleic acid in tear and conjunctival secretions of a non-conjunctivitis COVID-19 patient with obstruction of common lacrimal duct.
Hu, Yaguang; Chen, Tianyan; Liu, Miaomiao; Zhang, Li; Wang, Fang; Zhao, Shuo; Liu, Hui; Xia, Han; Wang, Yawen; Li, Li.
  • Hu Y; Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Chen T; Department of Infectious Diseases, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Liu M; Department of Cardiac Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Zhang L; Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Wang F; Department of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Zhao S; Department of Surgery Intensive Care Unit, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Liu H; Biobank, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Xia H; Faculty of Electronic and Information Engineering, School of Automation Science and Engineering, Xi'an Jiaotong University, Xi'an, China.
  • Wang Y; Department of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • Li L; Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Acta Ophthalmol ; 98(8): 859-863, 2020 Dec.
Article in English | MEDLINE | ID: covidwho-260153
Semantic information from SemMedBD (by NLM)
1. Nucleic Acids PART_OF Virus
Subject
Nucleic Acids
Predicate
PART_OF
Object
Virus
2. Common Duct LOCATION_OF Obstruction
Subject
Common Duct
Predicate
LOCATION_OF
Object
Obstruction
3. conjunctiva LOCATION_OF Nucleic Acids
Subject
conjunctiva
Predicate
LOCATION_OF
Object
Nucleic Acids
4. Tears body substance LOCATION_OF Nucleic Acids
Subject
Tears body substance
Predicate
LOCATION_OF
Object
Nucleic Acids
5. Infected PROCESS_OF Patients
Subject
Infected
Predicate
PROCESS_OF
Object
Patients
6. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
7. Lacrimal duct LOCATION_OF Obstruction
Subject
Lacrimal duct
Predicate
LOCATION_OF
Object
Obstruction
8. Obstruction PROCESS_OF Patients
Subject
Obstruction
Predicate
PROCESS_OF
Object
Patients
9. Eye Specimen PART_OF Patients
Subject
Eye Specimen
Predicate
PART_OF
Object
Patients
10. Eye LOCATION_OF Swab
Subject
Eye
Predicate
LOCATION_OF
Object
Swab
11. Eye LOCATION_OF Obstruction
Subject
Eye
Predicate
LOCATION_OF
Object
Obstruction
12. Eye LOCATION_OF Disease
Subject
Eye
Predicate
LOCATION_OF
Object
Disease
13. Nucleic Acids PART_OF Virus
Subject
Nucleic Acids
Predicate
PART_OF
Object
Virus
14. Common Duct LOCATION_OF Obstruction
Subject
Common Duct
Predicate
LOCATION_OF
Object
Obstruction
15. conjunctiva LOCATION_OF Nucleic Acids
Subject
conjunctiva
Predicate
LOCATION_OF
Object
Nucleic Acids
16. Tears body substance LOCATION_OF Nucleic Acids
Subject
Tears body substance
Predicate
LOCATION_OF
Object
Nucleic Acids
17. Infected PROCESS_OF Patients
Subject
Infected
Predicate
PROCESS_OF
Object
Patients
18. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
19. Lacrimal duct LOCATION_OF Obstruction
Subject
Lacrimal duct
Predicate
LOCATION_OF
Object
Obstruction
20. Obstruction PROCESS_OF Patients
Subject
Obstruction
Predicate
PROCESS_OF
Object
Patients
21. Eye Specimen PART_OF Patients
Subject
Eye Specimen
Predicate
PART_OF
Object
Patients
22. Eye LOCATION_OF Swab
Subject
Eye
Predicate
LOCATION_OF
Object
Swab
23. Eye LOCATION_OF Obstruction
Subject
Eye
Predicate
LOCATION_OF
Object
Obstruction
24. Eye LOCATION_OF Disease
Subject
Eye
Predicate
LOCATION_OF
Object
Disease
ABSTRACT

BACKGROUND:

The current outbreak of COVID-19 has spread rapidly all over the world. Respiratory droplets and contaction with infected patients are the two major transmission routes. However, the value of tear virus nucleic acid is still not clear. We dynamic detected the SARS-CoV-2 in eye sample of one COVID-19 patient with obstruction of common lacrimal ducts.

METHODS:

Besides the routine examination, nasopharyngeal and eye swab were continuously measured by polymerase chain reaction assay and next-generation sequencing (NGS). Gene detection was performed for drug use guidance, and flow cytometry was performed to analyse the lymphocyte subsets.

RESULTS:

Nasopharyngeal swabs were positive for 22 days, but eye swabs were still continuously positive for 2 weeks after nasopharyngeal swabs turned negative. The low level of lymphocyte and the high level IL-6 lasted for almost 4 weeks, then became near normal. Next-generation sequencing (NGS) confirmed the existing of SARS-CoV-2, HSV1 and HHV6B virus nucleic acid. The gene detection for drug use guidance showed the genetic locus ABCB1 (3435T>C) rs1045642 belonged to type CC and it mean the efficiency of lopinavir-ritonavir would be significantly decreased. The flow cytometry of lymphocyte subsets showed PD-1+  CD95+ cells was accounting for 94.8% in CD3+  CD8+ T subset and for 94.8% in CD3+  TCRγδ+ T subset.

CONCLUSIONS:

As obstruction of common lacrimal duct, positively detection in one eye for 2 weeks more after nasopharyngeal swab became negative. More eye swabs should be collected from COVID-19 patients, especially from those immunocompromised, those with eye symptoms and those had a history of ocular diseases.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Tears / Eye Infections, Viral / Herpesvirus 1, Human / Herpesvirus 6, Human / Conjunctiva / SARS-CoV-2 / COVID-19 / Lacrimal Duct Obstruction Type of study: Case report / Diagnostic study / Prognostic study Topics: Traditional medicine / Variants Language: English Journal: Acta Ophthalmol Journal subject: Ophthalmology Year: 2020 Document Type: Article Affiliation country: Aos.14456

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Tears / Eye Infections, Viral / Herpesvirus 1, Human / Herpesvirus 6, Human / Conjunctiva / SARS-CoV-2 / COVID-19 / Lacrimal Duct Obstruction Type of study: Case report / Diagnostic study / Prognostic study Topics: Traditional medicine / Variants Language: English Journal: Acta Ophthalmol Journal subject: Ophthalmology Year: 2020 Document Type: Article Affiliation country: Aos.14456