Identification of Coronavirus Isolated from a Patient in Korea with COVID-19.
Osong Public Health Res Perspect
; 11(1): 3-7, 2020 Feb.
Article
in English
| MEDLINE | ID: covidwho-2631
ABSTRACT
OBJECTIVES:
Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.METHODS:
Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.RESULTS:
The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.CONCLUSION:
SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Language:
English
Journal:
Osong Public Health Res Perspect
Year:
2020
Document Type:
Article
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