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Reverse-Transcription Recombinase-Aided Amplification Assay for Rapid Detection of the 2019 Novel Coronavirus (SARS-CoV-2).
Xue, Guanhua; Li, Shaoli; Zhang, Weiwei; Du, Bing; Cui, Jinghua; Yan, Chao; Huang, Lei; Chen, Lu; Zhao, Linqing; Sun, Yu; Li, Nannan; Zhao, Hanqing; Feng, Yanling; Wang, Zhimin; Liu, Shiyu; Zhang, Qun; Xie, Xianghui; Liu, Di; Yao, Hailan; Yuan, Jing.
  • Xue G; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Li S; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Zhang W; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Du B; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Cui J; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Yan C; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Huang L; Treatment and Research Center for Infectious Diseases, the Fifth Medical Center of PLA General Hospital, Beijing 100039, P. R. China.
  • Chen L; Beijing Macro&Micro-Test Bio-Tech Company, Limited, Beijing 101300, P. R. China.
  • Zhao L; Department of Virology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Sun Y; Department of Virology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Li N; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Zhao H; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Feng Y; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Wang Z; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center, Shanghai 201203, China.
  • Liu S; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Zhang Q; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Xie X; Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Liu D; Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, P. R. China.
  • Yao H; Department of Biochemistry and Immunology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
  • Yuan J; Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, P. R. China.
Anal Chem ; 92(14): 9699-9705, 2020 07 21.
Article in English | MEDLINE | ID: covidwho-342681
ABSTRACT
A novel coronavirus (SARS-CoV-2) was recently identified in patients with acute respiratory disease and spread quickly worldwide. A specific and rapid diagnostic method is important for early identification. The reverse-transcription recombinase-aided amplification (RT-RAA) assay is a rapid detection method for several pathogens. Assays were performed within 5-15 min as a one-step single tube reaction at 39 °C. In this study, we established two RT-RAA assays for the S and orf1ab gene of SARS-CoV-2 using clinical specimens for validation. The analytical sensitivity of the RT-RAA assay was 10 copies for the S and one copy for the orf1ab gene per reaction. Cross-reactions were not observed with any of the other respiratory pathogens. A 100% agreement between the RT-RAA and real-time PCR assays was accomplished after testing 120 respiratory specimens. These results demonstrate that the proposed RT-RAA assay will be beneficial as it is a faster, more sensitive, and more specific tool for the detection of SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Polymerase Chain Reaction / Coronavirus Infections / Nucleic Acid Amplification Techniques / Betacoronavirus Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Anal Chem Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Polymerase Chain Reaction / Coronavirus Infections / Nucleic Acid Amplification Techniques / Betacoronavirus Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Anal Chem Year: 2020 Document Type: Article