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SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
Lukassen, Soeren; Chua, Robert Lorenz; Trefzer, Timo; Kahn, Nicolas C; Schneider, Marc A; Muley, Thomas; Winter, Hauke; Meister, Michael; Veith, Carmen; Boots, Agnes W; Hennig, Bianca P; Kreuter, Michael; Conrad, Christian; Eils, Roland.
  • Lukassen S; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
  • Chua RL; Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.
  • Trefzer T; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
  • Kahn NC; Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.
  • Schneider MA; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
  • Muley T; Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.
  • Winter H; Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.
  • Meister M; Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.
  • Veith C; Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.
  • Boots AW; Translational Research Unit, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.
  • Hennig BP; Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.
  • Kreuter M; Translational Research Unit, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.
  • Conrad C; Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.
  • Eils R; Department of Thoracic Surgery, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.
EMBO J ; 39(10): e105114, 2020 05 18.
Article in English | MEDLINE | ID: covidwho-380778
ABSTRACT
The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bronchi / Serine Endopeptidases / Gene Expression / Peptidyl-Dipeptidase A / Single-Cell Analysis / Lung Type of study: Observational study Limits: Adult / Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal: EMBO J Year: 2020 Document Type: Article Affiliation country: Embj.20105114

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bronchi / Serine Endopeptidases / Gene Expression / Peptidyl-Dipeptidase A / Single-Cell Analysis / Lung Type of study: Observational study Limits: Adult / Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal: EMBO J Year: 2020 Document Type: Article Affiliation country: Embj.20105114