EZH2-mediated H3K27me3 inhibits ACE2 expression.
Biochem Biophys Res Commun
; 526(4): 947-952, 2020 06 11.
Article
in English
| MEDLINE | ID: covidwho-38740
ABSTRACT
The outbreak of corona virus disease 2019 (COVID-19) caused by SARS-CoV-2 infection is spreading globally and quickly, leading to emerging health issues. SARS-CoV-2 enters into and infects host cells through its spike glycoprotein recognizing the cell receptor Angiotensin-converting enzyme II (ACE2). Here, we noticed that ACE2 was further enhanced by SARS-CoV-2 infection. Human germ cells and early embryos express high level of ACE2. Notably, RNA-seq result showed that reduction of H3K27me3, but not H3K4/9/36me3, led to upregulation of Ace2 expression in mouse germ cell line GC-2. In agreement with this result, we found in human embryonic stem cells that ACE2 expression was significantly increased in absence of EZH2, the major enzyme catalyzing H3K27me3. ChIP-seq analysis further confirmed decrease of H3K27me3 signal and increase of H3K27ac signal at ACE2 promoter upon EZH2 knockout. Therefore, we propose that EZH2-mediated H3K27me3 at ACE2 promoter region inhibits ACE2 expression in mammalian cells. This regulatory pattern may also exist in other human cells and tissues. Our discovery provides clues for pathogenesis and targeted drug therapy towards ACE2 expression for prevention and adjuvant therapy of COVID-19.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
Gene Expression Regulation
/
Coronavirus Infections
/
Peptidyl-Dipeptidase A
/
Epigenesis, Genetic
/
Enhancer of Zeste Homolog 2 Protein
/
Betacoronavirus
Limits:
Animals
/
Humans
Language:
English
Journal:
Biochem Biophys Res Commun
Year:
2020
Document Type:
Article
Affiliation country:
J.bbrc.2020.04.010
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