Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2.
Curr Protoc Microbiol
; 57(1): ecpmc105, 2020 06.
Article
in English
| MEDLINE | ID: covidwho-437154
ABSTRACT
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causal agent of COronaVIrus Disease-19 (COVID-19), an atypical pneumonia-like syndrome that emerged in December 2019. While SARS-CoV-2 titers can be measured by detection of viral nucleic acid, this method is unable to quantitate infectious virions. Measurement of infectious SARS-CoV-2 can be achieved by tissue culture infectious dose-50 (TCID50 ), which detects the presence or absence of cytopathic effect in cells infected with serial dilutions of a virus specimen. However, this method only provides a qualitative infectious virus titer. Plaque assays are a quantitative method of measuring infectious SARS-CoV-2 by quantifying the plaques formed in cell culture upon infection with serial dilutions of a virus specimen. As such, plaque assays remain the gold standard in quantifying concentrations of replication-competent lytic virions. Here, we describe two detailed plaque assay protocols to quantify infectious SARS-CoV-2 using different overlay and staining methods. Both methods have several advantages and disadvantages, which can be considered when choosing the procedure best suited for each laboratory. These assays can be used for several research purposes, including titration of virus stocks produced from infected cell supernatant and, with further optimization, quantification of SARS-CoV-2 in specimens collected from infected animals. © 2019 The Authors. Basic Protocol SARS-CoV-2 plaque assay using a solid double overlay method Alternate Protocol SARS-CoV-2 plaque assay using a liquid overlay and fixation-staining method.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viral Plaque Assay
/
Clinical Protocols
/
Betacoronavirus
Type of study:
Prognostic study
/
Qualitative research
Limits:
Animals
/
Humans
Language:
English
Journal:
Curr Protoc Microbiol
Year:
2020
Document Type:
Article
Affiliation country:
Cpmc.105
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