Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients.
Ocul Immunol Inflamm
; 28(5): 721-725, 2020 Jul 03.
Article
in English
| MEDLINE | ID: covidwho-437299
ABSTRACT
PURPOSE:
To report the presence of viral ribonucleic acid (RNA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human retina in deceased patients with confirmed novel coronavirus disease 2019 (COVID-19). PATIENTS ANDMETHODS:
Fourteen eyes of 14 deceased patients with confirmed COVID-19 disease were enucleated during autopsy. A sample of human retina was secured and fixed in RNAlater™. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect three different viral RNA sequences (RdRp-gene, E-gene and Orf1 gene) of SARS-CoV-2.RESULTS:
In three out of 14 eyes SARS-CoV-2 viral RNA was detected in the retina of deceased COVID-19 patients. As analysis for three different sequences (RdRp-gene, E-gene and Orf1 gene) revealed positive results in RT-PCR, the existence of SARS-CoV-2 viral RNA in human retina is proven according to the standards of the World-Health-Organization.CONCLUSION:
Viral RNA of SARS-CoV-2 is detectable in the retina of COVID-19 patients.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
Retinal Diseases
/
Eye Infections, Viral
/
Coronavirus Infections
/
Betacoronavirus
Type of study:
Cohort study
/
Diagnostic study
/
Observational study
/
Prognostic study
Topics:
Vaccines
Limits:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
English
Journal:
Ocul Immunol Inflamm
Journal subject:
Allergy and Immunology
/
Ophthalmology
Year:
2020
Document Type:
Article
Affiliation country:
09273948.2020.1770301
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