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Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Konrad, Regina; Eberle, Ute; Dangel, Alexandra; Treis, Bianca; Berger, Anja; Bengs, Katja; Fingerle, Volker; Liebl, Bernhard; Ackermann, Nikolaus; Sing, Andreas.
  • Konrad R; Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Eberle U; These authors contributed equally to this work.
  • Dangel A; These authors contributed equally to this work.
  • Treis B; Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Berger A; Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Bengs K; These authors contributed equally to this work.
  • Fingerle V; Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Liebl B; Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Ackermann N; Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Sing A; Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
Euro Surveill ; 25(9)2020 03.
Article in English | MEDLINE | ID: covidwho-4532
ABSTRACT
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Prognostic study / Qualitative research Topics: Vaccines Limits: Humans Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: 1560-7917.ES.2020.25.9.2000173

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Prognostic study / Qualitative research Topics: Vaccines Limits: Humans Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: 1560-7917.ES.2020.25.9.2000173