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Serological immunochromatographic approach in diagnosis with SARS-CoV-2 infected COVID-19 patients.
Pan, Yunbao; Li, Xinran; Yang, Gui; Fan, Junli; Tang, Yueting; Zhao, Jin; Long, Xinghua; Guo, Shuang; Zhao, Ziwu; Liu, Yinjuan; Hu, Hanning; Xue, Han; Li, Yirong.
  • Pan Y; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Li X; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
  • Yang G; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Fan J; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Tang Y; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Zhao J; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Long X; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Guo S; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Zhao Z; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Liu Y; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Hu H; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China.
  • Xue H; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China. Electronic address: 58187532@qq.com.
  • Li Y; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No.169 Donghu Road, Wuhan, Hubei, China. Electronic address: 93419136@qq.com.
J Infect ; 81(1): e28-e32, 2020 07.
Article in English | MEDLINE | ID: covidwho-46420
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
Semantic information from SemMedBD (by NLM)
1. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
2. Ambulatory Care Facilities LOCATION_OF Diagnosis
Subject
Ambulatory Care Facilities
Predicate
LOCATION_OF
Object
Diagnosis
3. Immunochromatography USES Gold Colloid
Subject
Immunochromatography
Predicate
USES
Object
Gold Colloid
4. Gold Colloid INTERACTS_WITH CD40LG wt Allele|CD40LG
Subject
Gold Colloid
Predicate
INTERACTS_WITH
Object
CD40LG wt Allele|CD40LG
5. Immunochromatographic Assay USES Detection
Subject
Immunochromatographic Assay
Predicate
USES
Object
Detection
6. Immunochromatographic Assay USES CD40LG wt Allele|CD40LG
Subject
Immunochromatographic Assay
Predicate
USES
Object
CD40LG wt Allele|CD40LG
7. Immunochromatographic Assay DIAGNOSES Confirmed case
Subject
Immunochromatographic Assay
Predicate
DIAGNOSES
Object
Confirmed case
8. Immunochromatographic Assay DIAGNOSES 2019 novel coronavirus
Subject
Immunochromatographic Assay
Predicate
DIAGNOSES
Object
2019 novel coronavirus
9. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
10. Ambulatory Care Facilities LOCATION_OF Diagnosis
Subject
Ambulatory Care Facilities
Predicate
LOCATION_OF
Object
Diagnosis
11. Immunochromatography USES Gold Colloid
Subject
Immunochromatography
Predicate
USES
Object
Gold Colloid
12. Gold Colloid INTERACTS_WITH CD40LG wt Allele|CD40LG
Subject
Gold Colloid
Predicate
INTERACTS_WITH
Object
CD40LG wt Allele|CD40LG
13. Immunochromatographic Assay USES Detection
Subject
Immunochromatographic Assay
Predicate
USES
Object
Detection
14. Immunochromatographic Assay USES CD40LG wt Allele|CD40LG
Subject
Immunochromatographic Assay
Predicate
USES
Object
CD40LG wt Allele|CD40LG
15. Immunochromatographic Assay DIAGNOSES Confirmed case
Subject
Immunochromatographic Assay
Predicate
DIAGNOSES
Object
Confirmed case
16. Immunochromatographic Assay DIAGNOSES 2019 novel coronavirus
Subject
Immunochromatographic Assay
Predicate
DIAGNOSES
Object
2019 novel coronavirus
ABSTRACT
An outbreak of new coronavirus SARS-CoV-2 was occurred in Wuhan, China and rapidly spread to other cities and nations. The standard diagnostic approach that widely adopted in the clinic is nucleic acid detection by real-time RT-PCR. However, the false-negative rate of the technique is unneglectable and serological methods are urgently warranted. Here, we presented the colloidal gold-based immunochromatographic (ICG) strip targeting viral IgM or IgG antibody and compared it with real-time RT-PCR. The sensitivity of ICG assay with IgM and IgG combinatorial detection in nucleic acid confirmed cases were 11.1%, 92.9% and 96.8% at the early stage (1-7 days after onset), intermediate stage (8-14 days after onset), and late stage (more than 15 days), respectively. The ICG detection capacity in nucleic acid-negative suspected cases was 43.6%. In addition, the concordance of whole blood samples and plasma showed Cohen's kappa value of 0.93, which represented the almost perfect agreement between two types of samples. In conclusion, serological ICG strip assay in detecting SARS-CoV-2 infection is both sensitive and consistent, which is considered as an excellent supplementary approach in clinical application.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoassay / Serologic Tests / Coronavirus Infections / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study Topics: Long Covid Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Infect Year: 2020 Document Type: Article Affiliation country: J.jinf.2020.03.051

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoassay / Serologic Tests / Coronavirus Infections / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study Topics: Long Covid Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Infect Year: 2020 Document Type: Article Affiliation country: J.jinf.2020.03.051