Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro.
Virol J
; 17(1): 71, 2020 06 03.
Article
in English
| MEDLINE | ID: covidwho-505653
ABSTRACT
BACKGROUND:
Porcine epidemic diarrhea virus (PEDV) of the family Coronaviridae has caused substantial economic losses in the swine husbandry industry. There's currently no specific drug available for treatment of coronaviruses or PEDV.METHOD:
In the current study, we use coronavirus PEDV as a model to study antiviral agents. Briefly, a fusion inhibitor tHR2, recombinant lentivirus-delivered shRNAs targeted to conserved M and N sequences, homoharringtonine (HHT), and hydroxychloroquine (HCQ) were surveyed for their antiviral effects.RESULTS:
Treatment with HCQ at 50 µM and HHT at 150 nM reduced virus titer in TCID50 by 30 and 3.5 fold respectively, and the combination reduced virus titer in TCID50 by 200 fold.CONCLUSION:
Our report demonstrates that the combination of HHT and HCQ exhibited higher antiviral activity than either HHT or HCQ exhibited. The information may contribute to the development of antiviral strategies effective in controlling PEDV infection.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Antiviral Agents
/
RNA, Small Interfering
/
Porcine epidemic diarrhea virus
/
Homoharringtonine
/
Hydroxychloroquine
Type of study:
Observational study
Limits:
Animals
Language:
English
Journal:
Virol J
Journal subject:
Virology
Year:
2020
Document Type:
Article
Affiliation country:
S12985-020-01342-w
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