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Procedures for Flow Cytometry-Based Sorting of Unfixed Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infected Cells and Other Infectious Agents.
Reifel, Kristen M; Swan, Brandon K; Jellison, Evan R; Ambrozak, David; Baijer, Jan; Nguyen, Richard; Monard, Simon; Lyon, Geoffrey; Fontes, Benjamin; Perfetto, Stephen P.
  • Reifel KM; National Biodefense Analysis and Countermeasures Center, Frederick, Maryland, USA.
  • Swan BK; National Biodefense Analysis and Countermeasures Center, Frederick, Maryland, USA.
  • Jellison ER; Department of Immunology, UConn Health, Farmington, Connecticut, USA.
  • Ambrozak D; Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA.
  • Baijer J; CEA-DSV-IRCM, Fontenay-aux-Roses, France.
  • Nguyen R; Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA.
  • Monard S; Walter and Eliza Hall Institute, Parkville, Victoria, Australia.
  • Lyon G; Yale University Flow Cytometry Facility, New Haven, Connecticut, USA.
  • Fontes B; Yale University Environmental Health and Safety Office, New Haven, Connecticut, USA.
  • Perfetto SP; Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA.
Cytometry A ; 97(7): 674-680, 2020 07.
Article in English | MEDLINE | ID: covidwho-505930
ABSTRACT
In response to the recent COVID-19 pandemic, many laboratories are involved in research supporting SARS-CoV-2 vaccine development and clinical trials. Flow cytometry laboratories will be responsible for a large part of this effort by sorting unfixed antigen-specific lymphocytes. Therefore, it is critical and timely that we have an understanding of risk assessment and established procedures of infectious cell sorting. Here we present procedures covering the biosafety aspects of sorting unfixed SARS-CoV-2-infected cells and other infectious agents of similar risk level. These procedures follow the ISAC Biosafety Committee guidelines and were recently approved by the National Institutes of Health Institutional Biosafety Committee for sorting SARS-CoV-2-infected cells. © 2020 International Society for Advancement of Cytometry.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Specimen Handling / Coronavirus Infections / Containment of Biohazards / Pandemics / Flow Cytometry / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Cytometry A Year: 2020 Document Type: Article Affiliation country: Cyto.a.24040

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Specimen Handling / Coronavirus Infections / Containment of Biohazards / Pandemics / Flow Cytometry / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Cytometry A Year: 2020 Document Type: Article Affiliation country: Cyto.a.24040