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Genomic Sequencing and Analysis of Eight Camel-Derived Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Isolates in Saudi Arabia.
Al-Shomrani, Badr M; Manee, Manee M; Alharbi, Sultan N; Altammami, Mussad A; Alshehri, Manal A; Nassar, Majed S; Bakhrebah, Muhammed A; Al-Fageeh, Mohamed B.
  • Al-Shomrani BM; National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
  • Manee MM; National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
  • Alharbi SN; Institute of Bioinformatics, University of Georgia, Athens, GA 30602, USA.
  • Altammami MA; National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
  • Alshehri MA; National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
  • Nassar MS; National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
  • Bakhrebah MA; National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
  • Al-Fageeh MB; National Centre for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
Viruses ; 12(6)2020 06 03.
Article in English | MEDLINE | ID: covidwho-532726
ABSTRACT
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory illness in humans; the second-largest and most deadly outbreak to date occurred in Saudi Arabia. The dromedary camel is considered a possible host of the virus and also to act as a reservoir, transmitting the virus to humans. Here, we studied evolutionary relationships for 31 complete genomes of betacoronaviruses, including eight newly sequenced MERS-CoV genomes isolated from dromedary camels in Saudi Arabia. Through bioinformatics tools, we also used available sequences and 3D structure of MERS-CoV spike glycoprotein to predict MERS-CoV epitopes and assess antibody binding affinity. Phylogenetic analysis showed the eight new sequences have close relationships with existing strains detected in camels and humans in Arabian Gulf countries. The 2019-nCov strain appears to have higher homology to both bat coronavirus and SARS-CoV than to MERS-CoV strains. The spike protein tree exhibited clustering of MERS-CoV sequences similar to the complete genome tree, except for one sequence from Qatar (KF961222). B cell epitope analysis determined that the MERS-CoV spike protein has 24 total discontinuous regions from which just six epitopes were selected with score values of >80%. Our results suggest that the virus circulates by way of camels crossing the borders of Arabian Gulf countries. This study contributes to finding more effective vaccines in order to provide long-term protection against MERS-CoV and identifying neutralizing antibodies.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Camelus / Coronavirus Infections / Spike Glycoprotein, Coronavirus / Middle East Respiratory Syndrome Coronavirus Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Country/Region as subject: Asia Language: English Year: 2020 Document Type: Article Affiliation country: V12060611

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Camelus / Coronavirus Infections / Spike Glycoprotein, Coronavirus / Middle East Respiratory Syndrome Coronavirus Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Country/Region as subject: Asia Language: English Year: 2020 Document Type: Article Affiliation country: V12060611