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Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples.
Viruses ; 12(6)2020 06 08.
Article in English | MEDLINE | ID: covidwho-574875
ABSTRACT
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.
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Full text: Available Collection: International databases Database: MEDLINE Type: Article Main subject: Pneumonia, Viral / Coronavirus Infections / Virus Inactivation / Betacoronavirus Subject: Pneumonia, Viral / Coronavirus Infections / Virus Inactivation / Betacoronavirus Language: English Year: 2020

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Full text: Available Collection: International databases Database: MEDLINE Type: Article Main subject: Pneumonia, Viral / Coronavirus Infections / Virus Inactivation / Betacoronavirus Subject: Pneumonia, Viral / Coronavirus Infections / Virus Inactivation / Betacoronavirus Language: English Year: 2020