Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies.

Okba, Nisreen M A; Widjaja, Ivy; Li, Wentao; GeurtsvanKessel, Corine H; Farag, Elmoubasher A B A; Al-Hajri, Mohammed; Park, Wan Beom; Oh, Myoung-Don; Reusken, Chantal B E M; Koopmans, Marion P G; Bosch, Berend-Jan; Haagmans, Bart L

Okba, Nisreen M A; Widjaja, Ivy; Li, Wentao; GeurtsvanKessel, Corine H; Farag, Elmoubasher A B A; Al-Hajri, Mohammed; Park, Wan Beom; Oh, Myoung-Don; Reusken, Chantal B E M; Koopmans, Marion P G; Bosch, Berend-Jan; Haagmans, Bart L.

Emerg Infect Dis ; 26(5): 1024-1027, 2020 May.

Article in English | MEDLINE | ID: covidwho-5763

ABSTRACT

ABSTRACT

We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.

Subject(s)

Antibodies, Viral/blood; Coronavirus Infections/diagnosis; Enzyme-Linked Immunosorbent Assay/methods; Immunoassay/methods; Middle East Respiratory Syndrome Coronavirus/immunology; Serologic Tests/methods; Antibodies, Neutralizing/blood; Coronavirus Infections/blood; Coronavirus Infections/immunology; Coronavirus Infections/virology; Humans; Species Specificity

Keywords

MERS; MERS-CoV; Middle East respiratory syndrome; Middle East respiratory syndrome coronavirus; antibodies; dromedary; hemagglutination; human; lumazine synthase; nanoparticle; neutralization; respiratory infections; serology; spike; viruses; zoonoses

Fulltext

XML

PubMed Links

Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: Enzyme-Linked Immunosorbent Assay / Immunoassay / Serologic Tests / Coronavirus Infections / Middle East Respiratory Syndrome Coronavirus / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Emerg Infect Dis Journal subject: Communicable Diseases Year: 2020 Document Type: Article

Similar

MEDLINE

LILACS

LIS

Fulltext

XML

PubMed Links

Search on Google