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Combination of serological total antibody and RT-PCR test for detection of SARS-COV-2 infections.
Wang, Pei.
  • Wang P; Department of Laboratory Medicine, The First People's Hospital of Jingmen, Hubei Province, 448000, China. Electronic address: peiwwien@yahoo.com.
J Virol Methods ; 283: 113919, 2020 09.
Article in English | MEDLINE | ID: covidwho-598595
ABSTRACT
The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X2 = 5.16, P < 0.05), and similar to that of total antibody tests (X2 = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Serologic Tests / Coronavirus Infections / Real-Time Polymerase Chain Reaction / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Observational study Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged / Young adult Country/Region as subject: Asia Language: English Journal: J Virol Methods Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Serologic Tests / Coronavirus Infections / Real-Time Polymerase Chain Reaction / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Observational study Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged / Young adult Country/Region as subject: Asia Language: English Journal: J Virol Methods Year: 2020 Document Type: Article