Quality of Ribonucleic Acid Extraction for Real-Time Reverse Transcription-PCR (rRT-PCR) of SARS-CoV-2: Importance of Internal Control Monitoring.

Lee, Yeon Joo; Lim, Youngeun; Hur, Kyu Wha; Sung, Heungsup; Kim, Mi-Na

Lee, Yeon Joo; Lim, Youngeun; Hur, Kyu Wha; Sung, Heungsup; Kim, Mi-Na.

Lee YJ; Konkuk University School of Medicine, Seoul, Korea.
Lim Y; Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
Hur KW; Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
Sung H; Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.
Kim MN; Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea.

Ann Lab Med ; 40(6): 490-492, 2020 11.

Article in English | MEDLINE | ID: covidwho-599918

Subject(s)

Betacoronavirus/genetics; Coronavirus Infections/diagnosis; Pneumonia, Viral/diagnosis; RNA, Viral/metabolism; Real-Time Polymerase Chain Reaction/standards; Betacoronavirus/isolation & purification; COVID-19; Coronavirus Infections/virology; Humans; Magnetics; Nasopharynx/virology; Pandemics; Pneumonia, Viral/virology; Quality Control; RNA, Viral/isolation & purification; RNA, Viral/standards; Reagent Kits, Diagnostic; Republic of Korea; SARS-CoV-2; Sputum/virology

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / RNA, Viral / Coronavirus Infections / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: Ann Lab Med Year: 2020 Document Type: Article

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