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Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020.
Muenchhoff, Maximilian; Mairhofer, Helga; Nitschko, Hans; Grzimek-Koschewa, Natascha; Hoffmann, Dieter; Berger, Annemarie; Rabenau, Holger; Widera, Marek; Ackermann, Nikolaus; Konrad, Regina; Zange, Sabine; Graf, Alexander; Krebs, Stefan; Blum, Helmut; Sing, Andreas; Liebl, Bernhard; Wölfel, Roman; Ciesek, Sandra; Drosten, Christian; Protzer, Ulrike; Boehm, Stephan; Keppler, Oliver T.
  • Muenchhoff M; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
  • Mairhofer H; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Nitschko H; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
  • Grzimek-Koschewa N; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Hoffmann D; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
  • Berger A; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Rabenau H; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
  • Widera M; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Ackermann N; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Konrad R; Institute of Virology, School of Medicine, Technical University Munich/Helmholtz Zentrum München, Munich, Germany.
  • Zange S; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Graf A; Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.
  • Krebs S; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Blum H; Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.
  • Sing A; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Liebl B; Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.
  • Wölfel R; Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Ciesek S; Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Drosten C; German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
  • Protzer U; Bundeswehr Institute of Microbiology, Munich, Germany.
  • Boehm S; Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University, Munich, Germany.
  • Keppler OT; Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University, Munich, Germany.
Euro Surveill ; 25(24)2020 06.
Article in English | MEDLINE | ID: covidwho-605372
ABSTRACT
Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Diagnostic Equipment / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: 1560-7917.ES.2020.25.24.2001057

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Diagnostic Equipment / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: 1560-7917.ES.2020.25.24.2001057