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A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2.
Chi, Xiangyang; Yan, Renhong; Zhang, Jun; Zhang, Guanying; Zhang, Yuanyuan; Hao, Meng; Zhang, Zhe; Fan, Pengfei; Dong, Yunzhu; Yang, Yilong; Chen, Zhengshan; Guo, Yingying; Zhang, Jinlong; Li, Yaning; Song, Xiaohong; Chen, Yi; Xia, Lu; Fu, Ling; Hou, Lihua; Xu, Junjie; Yu, Changming; Li, Jianmin; Zhou, Qiang; Chen, Wei.
  • Chi X; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Yan R; Key Laboratory of Structural Biology of Zhejiang Province, Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang Province, China.
  • Zhang J; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Zhang G; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Zhang Y; Key Laboratory of Structural Biology of Zhejiang Province, Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang Province, China.
  • Hao M; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Zhang Z; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Fan P; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Dong Y; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Yang Y; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Chen Z; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Guo Y; Key Laboratory of Structural Biology of Zhejiang Province, Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang Province, China.
  • Zhang J; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Li Y; Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Song X; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Chen Y; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Xia L; Key Laboratory of Structural Biology of Zhejiang Province, Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang Province, China.
  • Fu L; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Hou L; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Xu J; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Yu C; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
  • Li J; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China. cw0226@foxmail.com zhouqiang@westlake.edu.cn lijmqz@126.com.
  • Zhou Q; Key Laboratory of Structural Biology of Zhejiang Province, Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang Province, China. cw0226@foxmail.com zhouqiang@westlake.edu.cn lijmqz@126.com.
  • Chen W; Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China. cw0226@foxmail.com zhouqiang@westlake.edu.cn lijmqz@126.com.
Science ; 369(6504): 650-655, 2020 08 07.
Article in English | MEDLINE | ID: covidwho-610891
Semantic information from SemMedBD (by NLM)
1. Antibodie PART_OF C0086418
Subject
Antibodie
Predicate
PART_OF
Object
C0086418
2. M Protei PART_OF C5203676
Subject
M Protei
Predicate
PART_OF
Object
C5203676
3. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
4. Patients LOCATION_OF Monoclonal Antibodies
Subject
Patients
Predicate
LOCATION_OF
Object
Monoclonal Antibodies
5. Antibodies, Blocking PART_OF Homo sapiens
Subject
Antibodies, Blocking
Predicate
PART_OF
Object
Homo sapiens
6. M Protein, multiple myeloma PART_OF 2019 novel coronavirus
Subject
M Protein, multiple myeloma
Predicate
PART_OF
Object
2019 novel coronavirus
7. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
8. Patients LOCATION_OF Monoclonal Antibodies
Subject
Patients
Predicate
LOCATION_OF
Object
Monoclonal Antibodies
ABSTRACT
Developing therapeutics against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be guided by the distribution of epitopes, not only on the receptor binding domain (RBD) of the Spike (S) protein but also across the full Spike (S) protein. We isolated and characterized monoclonal antibodies (mAbs) from 10 convalescent COVID-19 patients. Three mAbs showed neutralizing activities against authentic SARS-CoV-2. One mAb, named 4A8, exhibits high neutralization potency against both authentic and pseudotyped SARS-CoV-2 but does not bind the RBD. We defined the epitope of 4A8 as the N-terminal domain (NTD) of the S protein by determining with cryo-eletron microscopy its structure in complex with the S protein to an overall resolution of 3.1 angstroms and local resolution of 3.3 angstroms for the 4A8-NTD interface. This points to the NTD as a promising target for therapeutic mAbs against COVID-19.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Betacoronavirus / Antibodies, Monoclonal / Antibodies, Viral Language: English Journal: Science Year: 2020 Document Type: Article Affiliation country: Science.abc6952

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Betacoronavirus / Antibodies, Monoclonal / Antibodies, Viral Language: English Journal: Science Year: 2020 Document Type: Article Affiliation country: Science.abc6952