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Room-temperature-storable PCR mixes for SARS-CoV-2 detection.
Xu, Jiasu; Wang, Jin; Zhong, Zecheng; Su, Xiaosong; Yang, Kunyu; Chen, Zhongfu; Zhang, Dongxu; Li, Tingdong; Wang, Yingbin; Zhang, Shiyin; Ge, Shengxiang; Zhang, Jun; Xia, Ningshao.
  • Xu J; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Wang J; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Zhong Z; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Su X; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Yang K; Xiamen International Travel Healthcare Center, Xiamen 361013, China.
  • Chen Z; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Zhang D; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Li T; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Wang Y; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Zhang S; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China. Electronic address: zhangshiyin@xmu.edu.cn.
  • Ge S; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China. Electronic address: sxge@xmu.edu.cn.
  • Zhang J; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
  • Xia N; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, China.
Clin Biochem ; 84: 73-78, 2020 Oct.
Article in English | MEDLINE | ID: covidwho-612445
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ABSTRACT

OBJECTIVES:

A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks.

METHODS:

Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity. Additive-supplemented PCR mixes were freeze-dried. The residual moisture of the freeze-dried PCR mixes was measured by Karl-Fischer titration.

RESULTS:

We found that the freeze-dried PCR mixes with ~1.2% residual moisture are optimal for storage, transport, and reconstitution. The sensitivity, specificity, and repeatability of the freeze-dried reagents were similar to those of freshly prepared, wet reagents. The freeze-dried mixes retained activity at room temperature (18 ~ 25 °C) for 28 days, and for 14 and 10 days when stored at 37 °C and 56 °C, respectively.

CONCLUSION:

The uptake of this approach will ease logistical challenges faced by transport networks and make more cold storage space available at diagnosis and hospital laboratories.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / DNA, Viral / Polymerase Chain Reaction / DNA Primers / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Clin Biochem Year: 2020 Document Type: Article Affiliation country: J.clinbiochem.2020.06.013

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / DNA, Viral / Polymerase Chain Reaction / DNA Primers / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Type of study: Diagnostic study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Clin Biochem Year: 2020 Document Type: Article Affiliation country: J.clinbiochem.2020.06.013