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LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins.
Haljasmägi, Liis; Remm, Anu; Rumm, Anna Pauliina; Krassohhina, Ekaterina; Sein, Hanna; Tamm, Anu; Kisand, Kai; Peterson, Pärt.
  • Haljasmägi L; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
  • Remm A; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
  • Rumm AP; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
  • Krassohhina E; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
  • Sein H; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
  • Tamm A; United Laboratories, Tartu University Hospital, Tartu, Estonia.
  • Kisand K; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
  • Peterson P; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
Eur J Immunol ; 50(8): 1234-1236, 2020 08.
Article in English | MEDLINE | ID: covidwho-614139
ABSTRACT
Profiling antibodies to SARS-CoV-2 can help to assess potential immune response after COVID-19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID-19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID-19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS-CoV-2 antigens.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Nucleocapsid Proteins / Pandemics / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Eur J Immunol Year: 2020 Document Type: Article Affiliation country: Eji.202048715

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Nucleocapsid Proteins / Pandemics / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Eur J Immunol Year: 2020 Document Type: Article Affiliation country: Eji.202048715