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An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening.
Amanat, Fatima; White, Kris M; Miorin, Lisa; Strohmeier, Shirin; McMahon, Meagan; Meade, Philip; Liu, Wen-Chun; Albrecht, Randy A; Simon, Viviana; Martinez-Sobrido, Luis; Moran, Thomas; García-Sastre, Adolfo; Krammer, Florian.
  • Amanat F; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York.
  • White KM; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Miorin L; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Strohmeier S; Global Health Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
  • McMahon M; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Meade P; Global Health Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Liu WC; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Albrecht RA; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Simon V; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Martinez-Sobrido L; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Moran T; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • García-Sastre A; Global Health Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Krammer F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
Curr Protoc Microbiol ; 58(1): e108, 2020 09.
Article in English | MEDLINE | ID: covidwho-614201
ABSTRACT
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS-CoV-2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS-CoV-2 in vitro. © 2020 Wiley Periodicals LLC. Basic Protocol 1 Microneutralization assay to test inhibition of virus by antibodies (purified antibodies or serum/plasma) Basic Protocol 2 Screening of anti-SARS-CoV-2 compounds in vitro Support Protocol SARS-CoV-2 propagation.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Neutralization Tests / Drug Evaluation, Preclinical / Betacoronavirus / Antibodies, Viral Type of study: Prognostic study Topics: Traditional medicine / Vaccines Limits: Animals / Humans Language: English Journal: Curr Protoc Microbiol Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Neutralization Tests / Drug Evaluation, Preclinical / Betacoronavirus / Antibodies, Viral Type of study: Prognostic study Topics: Traditional medicine / Vaccines Limits: Animals / Humans Language: English Journal: Curr Protoc Microbiol Year: 2020 Document Type: Article