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Comparison between chemiluminescence and colloidal gold method in the detection of 2019 novel coronavirus specific antibodies and the value in COVID-19 diagnosis and treatment / 化学发光和胶体金法检测新型冠状病毒特异性抗体比较及其临床意义
Tang, Peng; Zhao, Ziwu; Liu, Yinjuan; Chen, Yawen; Peng, Dinghui; Wang, Dong; Li, Yirong; Xie, Wen.
  • Tang, Peng; Dept. of Clinical Laboratory. Wuhan. China
  • Zhao, Ziwu; Dept. of Clinical Laboratory. Wuhan. China
  • Liu, Yinjuan; Dept. of Clinical Laboratory. Wuhan. China
  • Chen, Yawen; Dept. of Clinical Laboratory. Wuhan. China
  • Peng, Dinghui; Dept. of Clinical Laboratory. Wuhan. China
  • Wang, Dong; Dept. of Clinical Laboratory. Wuhan. China
  • Li, Yirong; Dept. of Clinical Laboratory. Wuhan. China
  • Xie, Wen; Dept. of Clinical Laboratory. Wuhan. China
Med. J. Wuhan Univ. ; 4(41): 517-520, 20200715.
Article in Chinese | WHO COVID, ELSEVIER | ID: covidwho-617504
ABSTRACT

Objective:

To compare the chemiluminescence and colloidal gold method in the detection of 2019 novel Coronavirus (2019-nCoV, or SARS-CoV-2) specific antibody and to evaluate the importance in the diagnosis and treatment of COVID-19.

Methods:

A total of 191 cases of diagnosed and suspected COVID-19 patients admitted in Febrary 2020 in Wuhan Leishenshan Hospital were included. The patients were divided into 2019-nCoV continuous positive group, 2019-nCoV positive to negative group, and 2019-nCoV suspect group according to the results of pharyngeal swab 2019-nCoV nucleic acid detection. In addition, 27 patients without COVID-19 were set as control group to test the specificity. The serum samples were collected, and colloidal gold and chemiluminescence method were used to detect total antibodies (IgM+IgG) and IgM. SPSS 24.0 was used to compare the difference in antibody detection rates between the two methods.

Results:

The specificity of total antibodies (IgM+IgG) and IgM detection was 100%. Two methods of total antibody detection had no significant difference in continuous positive group (P=1.00). In positive to negative group and suspect group, the positive detection rate of chemiluminescence method was significantly higher than colloidal gold method (95.2% vs 76.2% and 94.9% vs 70.5%, respectively, both P<0.05). The chemiluminescence method showed no significant difference between the three groups in IgM detection (P>0.05).

Conclusion:

The detection of 2019-nCoV specific antibody can be an important supplemental method to pharyngeal swab 2019-nCoV nucleic acid detection during the diagnosis and treatment of COVID-19. The chemiluminescence method is better than colloidal gold method in the detection of total antibody. The IgM detection using chemiluminescence method is suitable for all stages of the disease.

Full text: Available Collection: International databases Database: WHO COVID / ELSEVIER Type of study: Diagnostic study / Observational study / Randomized controlled trials Language: Chinese Journal: Med. J. Wuhan Univ. Year: 2020 Document Type: Article Institution/Affiliation country: Dept. of Clinical Laboratory/China

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Full text: Available Collection: International databases Database: WHO COVID / ELSEVIER Type of study: Diagnostic study / Observational study / Randomized controlled trials Language: Chinese Journal: Med. J. Wuhan Univ. Year: 2020 Document Type: Article Institution/Affiliation country: Dept. of Clinical Laboratory/China