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Evaluation of SARS-CoV-2 viral RNA in fecal samples.
Mesoraca, Alvaro; Margiotti, Katia; Viola, Antonella; Cima, Antonella; Sparacino, Davide; Giorlandino, Claudio.
  • Mesoraca A; ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.
  • Margiotti K; ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy. katia.margiotti@artemisia.it.
  • Viola A; ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.
  • Cima A; ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.
  • Sparacino D; ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.
  • Giorlandino C; ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.
Virol J ; 17(1): 86, 2020 06 30.
Article in English | MEDLINE | ID: covidwho-618211
ABSTRACT
The need for timely establishment of a complete diagnostic protocol of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is demanded worldwide. We selected 15 positive novel coronavirus disease 19 (COVID-19) patients with mild or no symptom. Initially, fecal samples were negative in the 67% (10/15) of the cases, while 33% (5/10) of the cases were positive. After serial virus RNA testing, 73% (11/15) of the cases resulted positive to fecal specimens. In particular, 15 days after the first positive respiratory specimens test, 6 fecal specimens became positive for SARS-CoV-2 RNA, while 13 respiratory test returned negative result. In conclusion, qRT-PCR assays of fecal specimens, is an important step to control infection, suggesting that samples remained positive for SARS-CoV-2 RNA longer time then respiratory tract samples. Our results enhance the recent knowledge on this emerging infectious disease and offer suggestions for a more complete diagnostic strategy.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Feces / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Observational study Topics: Long Covid Limits: Female / Humans / Male Language: English Journal: Virol J Journal subject: Virology Year: 2020 Document Type: Article Affiliation country: S12985-020-01359-1

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Feces / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Observational study Topics: Long Covid Limits: Female / Humans / Male Language: English Journal: Virol J Journal subject: Virology Year: 2020 Document Type: Article Affiliation country: S12985-020-01359-1