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Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2.
Hu, Xiumei; An, Taixue; Situ, Bo; Hu, Yuhai; Ou, Zihao; Li, Qiang; He, Xiaojing; Zhang, Ye; Tian, Peifu; Sun, Dehua; Rui, Yongyu; Wang, Qian; Ding, Dan; Zheng, Lei.
  • Hu X; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • An T; Guangdong Medical Aid Team for Hubei Province, Wuhan, China.
  • Situ B; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Hu Y; Guangdong Medical Aid Team for Hubei Province, Wuhan, China.
  • Ou Z; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Li Q; Department of Medicine Laboratory, Hankou Hospital of Wuhan, Wuhan, China.
  • He X; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Zhang Y; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Tian P; Guangdong Medical Aid Team for Hubei Province, Wuhan, China.
  • Sun D; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Rui Y; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Wang Q; Department of Medicine Laboratory, Hankou Hospital of Wuhan, Wuhan, China.
  • Ding D; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Zheng L; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
J Clin Lab Anal ; 34(9): e23411, 2020 Sep.
Article in English | MEDLINE | ID: covidwho-624944
Preprint
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ABSTRACT

BACKGROUND:

The detection of serum antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is emerging as a new tool for the coronavirus disease 2019 (COVID-19) diagnosis. Since many coronaviruses are sensitive to heat, heating inactivation of samples at 56°C prior to testing is considered a possible method to reduce the risk of transmission, but the effect of heating on the measurement of SARS-CoV-2 antibodies is still unclear.

METHODS:

By comparing the levels of SARS-CoV-2 antibodies before and after heat inactivation of serum at 56°C for 30 minutes using a quantitative fluorescence immunochromatographic assay

RESULTS:

We showed that heat inactivation significantly interferes with the levels of antibodies to SARS-CoV-2. The IgM levels of all the 34 serum samples (100%) from COVID-19 patients decreased by an average level of 53.56%. The IgG levels were decreased in 22 of 34 samples (64.71%) by an average level of 49.54%. Similar changes can also be observed in the non-COVID-19 disease group (n = 9). Of note, 44.12% of the detected IgM levels were dropped below the cutoff value after heating, suggesting heat inactivation can lead to false-negative results of these samples.

CONCLUSION:

Our results indicate that heat inactivation of serum at 56°C for 30 minutes interferes with the immunoanalysis of antibodies to SARS-CoV-2. Heat inactivation prior to immunoanalysis is not recommended, and the possibility of false-negative results should be considered if the sample was pre-inactivated by heating.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoassay / Coronavirus Infections / Serum / Betacoronavirus / Hot Temperature / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2020 Document Type: Article Affiliation country: Jcla.23411

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoassay / Coronavirus Infections / Serum / Betacoronavirus / Hot Temperature / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2020 Document Type: Article Affiliation country: Jcla.23411