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A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition.
Dieterle, M Eugenia; Haslwanter, Denise; Bortz, Robert H; Wirchnianski, Ariel S; Lasso, Gorka; Vergnolle, Olivia; Abbasi, Shawn A; Fels, J Maximilian; Laudermilch, Ethan; Florez, Catalina; Mengotto, Amanda; Kimmel, Duncan; Malonis, Ryan J; Georgiev, George; Quiroz, Jose; Barnhill, Jason; Pirofski, Liise-Anne; Daily, Johanna P; Dye, John M; Lai, Jonathan R; Herbert, Andrew S; Chandran, Kartik; Jangra, Rohit K.
  • Dieterle ME; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Haslwanter D; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Bortz RH; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Wirchnianski AS; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Lasso G; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Vergnolle O; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Abbasi SA; U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.
  • Fels JM; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Laudermilch E; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Florez C; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY 10996, USA.
  • Mengotto A; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Kimmel D; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Malonis RJ; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Georgiev G; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Quiroz J; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Barnhill J; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY 10996, USA.
  • Pirofski LA; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Daily JP; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Dye JM; U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.
  • Lai JR; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Herbert AS; U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA; The Geneva Foundation, 917 Pacific Avenue, Tacoma, WA 98402, USA. Electronic address: andrew.s.herbert4.ctr@mail.mil.
  • Chandran K; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address: kartik.chandran@einsteinmed.org.
  • Jangra RK; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address: rohit.jangra@einsteinmed.org.
Cell Host Microbe ; 28(3): 486-496.e6, 2020 09 09.
Article in English | MEDLINE | ID: covidwho-627576
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Vesicular stomatitis Indiana virus / Spike Glycoprotein, Coronavirus / Betacoronavirus Type of study: Prognostic study Topics: Traditional medicine / Vaccines Language: English Journal: Cell Host Microbe Journal subject: Microbiology Year: 2020 Document Type: Article Affiliation country: J.chom.2020.06.020

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Vesicular stomatitis Indiana virus / Spike Glycoprotein, Coronavirus / Betacoronavirus Type of study: Prognostic study Topics: Traditional medicine / Vaccines Language: English Journal: Cell Host Microbe Journal subject: Microbiology Year: 2020 Document Type: Article Affiliation country: J.chom.2020.06.020