Evolving sequence mutations in the Middle East Respiratory Syndrome Coronavirus (MERS-CoV).
J Infect Public Health
; 13(10): 1544-1550, 2020 Oct.
Article
in English
| MEDLINE | ID: covidwho-633894
ABSTRACT
BACKGROUND:
Middle East respiratory syndrome coronavirus (MERS-CoV) has continued to cause sporadic outbreaks of severe respiratory tract infection over the last 8 years.METHODS:
Complete genome sequencing using next-generation sequencing was performed for MERS-CoV isolates from cases that occurred in Riyadh between 2015 and 2019. Phylogenetic analysis and molecular mutational analysis were carried out to investigate disease severity.RESULTS:
A total of eight MERS-CoV isolates were subjected to complete genome sequencing. Phylogenetic analysis resulted in the assembly of 7/8 sequences within lineage 3 and one sequence within lineage 4 showing complex genomic recombination. The isolates contained a variety of unique amino acid substitutions in ORF1ab (41), the N protein (10), the S protein (9) and ORF4b (5).CONCLUSION:
Our study shows that MERS-CoV is evolving. The emergence of new variants carries the potential for increased virulence and could impose a challenge to the global health system. We recommend the sequencing every new MERS-CoV isolate to observe the changes in the virus and relate them to clinical outcomes.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
RNA, Viral
/
Coronavirus Infections
/
Middle East Respiratory Syndrome Coronavirus
/
Mutation
Type of study:
Prognostic study
/
Randomized controlled trials
Topics:
Variants
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Country/Region as subject:
Asia
Language:
English
Journal:
J Infect Public Health
Journal subject:
Communicable Diseases
/
Public Health
Year:
2020
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS