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Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics?
Viruses ; 12(7)2020 07 07.
Article in English | MEDLINE | ID: covidwho-639283
ABSTRACT
Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. We evaluated three heat inactivation protocols (56 °C-30 min, 60 °C-60 min and 92 °C-15 min) on SARS-CoV-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the European norm NF EN 14476-A2. Regardless of the protocol and the type of samples, a 4 Log10 TCID50 reduction was observed. However, samples containing viral loads > 6 Log10 TCID50 were still infectious after 56 °C-30 min and 60 °C-60 min, although infectivity was < 10 TCID50. The protocols 56 °C-30 min and 60 °C-60 min had little influence on the RNA copies detection, whereas 92 °C-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. Lastly, 56 °C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test, whereas a drastic decrease in neutralizing titers was observed.
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Full text: Available Collection: International databases Database: MEDLINE Type: Article Main subject: Pneumonia, Viral / Serologic Tests / Coronavirus Infections / Containment of Biohazards / Virus Inactivation / Betacoronavirus Subject: Pneumonia, Viral / Serologic Tests / Coronavirus Infections / Containment of Biohazards / Virus Inactivation / Betacoronavirus Type of study: Diagnostic study / Clinical Practice Guide Language: English Year: 2020

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Full text: Available Collection: International databases Database: MEDLINE Type: Article Main subject: Pneumonia, Viral / Serologic Tests / Coronavirus Infections / Containment of Biohazards / Virus Inactivation / Betacoronavirus Subject: Pneumonia, Viral / Serologic Tests / Coronavirus Infections / Containment of Biohazards / Virus Inactivation / Betacoronavirus Type of study: Diagnostic study / Clinical Practice Guide Language: English Year: 2020