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A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019.
Cai, Xue-Fei; Chen, Juan; Li Hu, Jie-; Long, Quan-Xin; Deng, Hai-Jun; Liu, Ping; Fan, Kai; Liao, Pu; Liu, Bei-Zhong; Wu, Gui-Cheng; Chen, Yao-Kai; Li, Zhi-Jie; Wang, Kun; Zhang, Xiao-Li; Tian, Wen-Guang; Xiang, Jiang-Lin; Du, Hong-Xin; Wang, Jing; Hu, Yuan; Tang, Ni; Lin, Yong; Ren, Ji-Hua; Huang, Lu-Yi; Wei, Jie; Gan, Chun-Yang; Chen, Yan-Meng; Gao, Qing-Zhu; Chen, A-Mei; He, Chang-Long; Wang, Dao-Xin; Hu, Peng; Zhou, Fa-Chun; Huang, Ai-Long; Wang, De-Qiang.
  • Cai XF; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Chen J; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Li Hu J; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Long QX; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Deng HJ; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Liu P; BioScience Co. LTD, Tianjin, China.
  • Fan K; PEG-Bio Biopharm Co. LTD., Chongqing, China.
  • Liao P; Laboratory Department, Chongqing People's Hospital, Chongqing, China.
  • Liu BZ; Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
  • Wu GC; Chongqing Three Gorges Central Hospital, Chongqing, China.
  • Chen YK; The Public Health Center, Chongqing, China.
  • Li ZJ; Laboratory Department, Chongqing People's Hospital, Chongqing, China.
  • Wang K; Laboratory Department, Chongqing People's Hospital, Chongqing, China.
  • Zhang XL; Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
  • Tian WG; Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
  • Xiang JL; Chongqing Three Gorges Central Hospital, Chongqing, China.
  • Du HX; Chongqing Three Gorges Central Hospital, Chongqing, China.
  • Wang J; The Public Health Center, Chongqing, China.
  • Hu Y; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Tang N; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Lin Y; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Ren JH; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Huang LY; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Wei J; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Gan CY; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Chen YM; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Gao QZ; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Chen AM; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • He CL; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Wang DX; Department of Respiration, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
  • Hu P; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Zhou FC; Department of Emergency, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
  • Huang AL; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
  • Wang DQ; Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
J Infect Dis ; 222(2): 189-193, 2020 06 29.
Article in English | MEDLINE | ID: covidwho-643587
Semantic information from SemMedBD (by NLM)
1. Chemiluminescence assay USES Peptides
Subject
Chemiluminescence assay
Predicate
USES
Object
Peptides
2. Coronavirus Infections CAUSES Pneumonia
Subject
Coronavirus Infections
Predicate
CAUSES
Object
Pneumonia
3. Severe Acute Respiratory Syndrome COEXISTS_WITH Disease
Subject
Severe Acute Respiratory Syndrome
Predicate
COEXISTS_WITH
Object
Disease
4. Immunoenzyme Procedure USES immunoglobulin G
Subject
Immunoenzyme Procedure
Predicate
USES
Object
immunoglobulin G
5. Immunoenzyme Procedure USES Peptides
Subject
Immunoenzyme Procedure
Predicate
USES
Object
Peptides
6. Immunoenzyme Procedure USES CD40LG wt Allele|CD40LG
Subject
Immunoenzyme Procedure
Predicate
USES
Object
CD40LG wt Allele|CD40LG
7. Serum ASSOCIATED_WITH Infected
Subject
Serum
Predicate
ASSOCIATED_WITH
Object
Infected
8. Infected PROCESS_OF Patients
Subject
Infected
Predicate
PROCESS_OF
Object
Patients
9. Serum PART_OF Patients
Subject
Serum
Predicate
PART_OF
Object
Patients
10. Confirmation PROCESS_OF Patients
Subject
Confirmation
Predicate
PROCESS_OF
Object
Patients
11. Patients LOCATION_OF Serum
Subject
Patients
Predicate
LOCATION_OF
Object
Serum
12. Serum LOCATION_OF CD40LG
Subject
Serum
Predicate
LOCATION_OF
Object
CD40LG
13. Chemiluminescence assay USES Peptides
Subject
Chemiluminescence assay
Predicate
USES
Object
Peptides
14. Coronavirus Infections CAUSES Pneumonia
Subject
Coronavirus Infections
Predicate
CAUSES
Object
Pneumonia
15. Severe Acute Respiratory Syndrome COEXISTS_WITH Disease
Subject
Severe Acute Respiratory Syndrome
Predicate
COEXISTS_WITH
Object
Disease
16. Immunoenzyme Procedure USES immunoglobulin G
Subject
Immunoenzyme Procedure
Predicate
USES
Object
immunoglobulin G
17. Immunoenzyme Procedure USES Peptides
Subject
Immunoenzyme Procedure
Predicate
USES
Object
Peptides
18. Immunoenzyme Procedure USES CD40LG wt Allele|CD40LG
Subject
Immunoenzyme Procedure
Predicate
USES
Object
CD40LG wt Allele|CD40LG
19. Serum ASSOCIATED_WITH Infected
Subject
Serum
Predicate
ASSOCIATED_WITH
Object
Infected
20. Infected PROCESS_OF Patients
Subject
Infected
Predicate
PROCESS_OF
Object
Patients
21. Serum PART_OF Patients
Subject
Serum
Predicate
PART_OF
Object
Patients
22. Confirmation PROCESS_OF Patients
Subject
Confirmation
Predicate
PROCESS_OF
Object
Patients
23. Patients LOCATION_OF Serum
Subject
Patients
Predicate
LOCATION_OF
Object
Serum
24. Serum LOCATION_OF CD40LG
Subject
Serum
Predicate
LOCATION_OF
Object
CD40LG
ABSTRACT

BACKGROUND:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel ß-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection.

METHODS:

In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2.

RESULTS:

To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively.

CONCLUSIONS:

Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Serologic Tests / Immunoenzyme Techniques / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: J Infect Dis Year: 2020 Document Type: Article Affiliation country: Infdis

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Serologic Tests / Immunoenzyme Techniques / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: J Infect Dis Year: 2020 Document Type: Article Affiliation country: Infdis