A virtual ELISA to quantitate COVID-19 antibodies in patient serum.
Biochem Mol Biol Educ
; 48(5): 467-468, 2020 09.
Article
in English
| MEDLINE | ID: covidwho-644443
ABSTRACT
Enzyme-linked immunosorbent assays (ELISAs) are used widely in biotechnology, pharmaceutical, and clinical medicine labs. At the same time, they appear to be underrepresented in chemistry and biochemistry curricula, even though their sensitivity, selectivity, and ease of use would argue for their widespread use. We describe here an online ELISA activity suitable for stand-alone use or in conjunction with an actual wet lab ELISA. Specifically, we offer real and mock data for a hypothetical ELISA to detect plasma antibodies to COVID-19 in infected patients who have had the disease. Much of the activity focuses on chemical and mathematical models to fit ELISA or any macromolecule/ligand binding data, a skill that addresses perhaps the most relevant and difficult learning goal of an ELISA experiment.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Education, Distance
/
Pandemics
/
COVID-19 Serological Testing
/
SARS-CoV-2
/
COVID-19
/
Models, Chemical
/
Antibodies, Viral
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Biochem Mol Biol Educ
Year:
2020
Document Type:
Article
Affiliation country:
Bmb.21403
Similar
MEDLINE
...
LILACS
LIS