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SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro.
Cipitelli, Marcio da Costa; Valentin, Elizabeth; da Cruz, Nadia Vaez Gonçalves; Nogueira, Tatiana Ls; de Melo, Elaine Cristina Amaro; da Silva, Rebeca Araujo; Serra, Marcelo M; Meriano, André L; Colares, Alberto Ml; Dornelas-Ribeiro, Marcos; Santos, Caleb Gm.
  • Cipitelli MDC; Laboratório de Biologia Molecular, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • Valentin E; Laboratório de Biodefesa, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • da Cruz NVG; Laboratório de Biodefesa, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • Nogueira TL; Laboratório de Genética, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • de Melo ECA; Laboratório de Biologia Molecular, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • da Silva RA; Laboratório de Biologia Molecular, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • Serra MM; Laboratório de Biodefesa, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • Meriano AL; Laboratório de Biologia Molecular, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • Colares AM; Laboratório de Biodefesa, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • Dornelas-Ribeiro M; Laboratório de Biologia Molecular, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
  • Santos CG; Laboratório de Biologia Molecular, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
Mem Inst Oswaldo Cruz ; 115: e200200, 2020.
Article in English | MEDLINE | ID: covidwho-646249
ABSTRACT
Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Betacoronavirus Type of study: Diagnostic study / Observational study Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem Inst Oswaldo Cruz Year: 2020 Document Type: Article Affiliation country: 0074-02760200200

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Betacoronavirus Type of study: Diagnostic study / Observational study Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem Inst Oswaldo Cruz Year: 2020 Document Type: Article Affiliation country: 0074-02760200200