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Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles.
Moitra, Parikshit; Alafeef, Maha; Dighe, Ketan; Frieman, Matthew B; Pan, Dipanjan.
  • Moitra P; Departments of Diagnostic Radiology and Nuclear Medicine and Pediatrics, Center for Blood Oxygen Transport and Hemostasis, University of Maryland Baltimore School of Medicine, Health Sciences Facility III, 670 West Baltimore Street, Baltimore, Maryland 21201, United States.
  • Alafeef M; Departments of Diagnostic Radiology and Nuclear Medicine and Pediatrics, Center for Blood Oxygen Transport and Hemostasis, University of Maryland Baltimore School of Medicine, Health Sciences Facility III, 670 West Baltimore Street, Baltimore, Maryland 21201, United States.
  • Dighe K; Bioengineering Department, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
  • Frieman MB; Biomedical Engineering Department, Jordan University of Science and Technology, Irbid 22110, Jordan.
  • Pan D; Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland Baltimore County, Interdisciplinary Health Sciences Facility, 1000 Hilltop Circle, Baltimore, Maryland 21250, United States.
ACS Nano ; 14(6): 7617-7627, 2020 06 23.
Article in English | MEDLINE | ID: covidwho-647565
Semantic information from SemMedBD (by NLM)
1. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
2. Phosphoproteins PART_OF Nucleocapsid
Subject
Phosphoproteins
Predicate
PART_OF
Object
Nucleocapsid
3. Assay USES Colorimetry
Subject
Assay
Predicate
USES
Object
Colorimetry
4. Assay DIAGNOSES COVID-19
Subject
Assay
Predicate
DIAGNOSES
Object
COVID-19
5. RNA Sequence PART_OF 2019 novel coronavirus
Subject
RNA Sequence
Predicate
PART_OF
Object
2019 novel coronavirus
6. Assay DIAGNOSES Present
Subject
Assay
Predicate
DIAGNOSES
Object
Present
7. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
8. Phosphoproteins PART_OF Nucleocapsid
Subject
Phosphoproteins
Predicate
PART_OF
Object
Nucleocapsid
9. Assay USES Colorimetry
Subject
Assay
Predicate
USES
Object
Colorimetry
10. Assay DIAGNOSES COVID-19
Subject
Assay
Predicate
DIAGNOSES
Object
COVID-19
11. RNA Sequence PART_OF 2019 novel coronavirus
Subject
RNA Sequence
Predicate
PART_OF
Object
2019 novel coronavirus
12. Assay DIAGNOSES Present
Subject
Assay
Predicate
DIAGNOSES
Object
Present
ABSTRACT
The current outbreak of the pandemic coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) demands its rapid, convenient, and large-scale diagnosis to downregulate its spread within as well as across the communities. But the reliability, reproducibility, and selectivity of majority of such diagnostic tests fail when they are tested either to a viral load at its early representation or to a viral gene mutated during its current spread. In this regard, a selective "naked-eye" detection of SARS-CoV-2 is highly desirable, which can be tested without accessing any advanced instrumental techniques. We herein report the development of a colorimetric assay based on gold nanoparticles (AuNPs), when capped with suitably designed thiol-modified antisense oligonucleotides (ASOs) specific for N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2, could be used for diagnosing positive COVID-19 cases within 10 min from the isolated RNA samples. The thiol-modified ASO-capped AuNPs agglomerate selectively in the presence of its target RNA sequence of SARS-CoV-2 and demonstrate a change in its surface plasmon resonance. Further, the addition of RNaseH cleaves the RNA strand from the RNA-DNA hybrid leading to a visually detectable precipitate from the solution mediated by the additional agglomeration among the AuNPs. The selectivity of the assay has been monitored in the presence of MERS-CoV viral RNA with a limit of detection of 0.18 ng/µL of RNA having SARS-CoV-2 viral load. Thus, the current study reports a selective and visual "naked-eye" detection of COVID-19 causative virus, SARS-CoV-2, without the requirement of any sophisticated instrumental techniques.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Pneumonia, Viral / Biosensing Techniques / Oligonucleotides, Antisense / Coronavirus Infections / Nucleocapsid Proteins / Metal Nanoparticles / Betacoronavirus Subject: Pneumonia, Viral / Biosensing Techniques / Oligonucleotides, Antisense / Coronavirus Infections / Nucleocapsid Proteins / Metal Nanoparticles / Betacoronavirus Type of study: Diagnostic study Language: English Journal: ACS Nano Clinical aspect: Diagnosis Year: 2020

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Pneumonia, Viral / Biosensing Techniques / Oligonucleotides, Antisense / Coronavirus Infections / Nucleocapsid Proteins / Metal Nanoparticles / Betacoronavirus Subject: Pneumonia, Viral / Biosensing Techniques / Oligonucleotides, Antisense / Coronavirus Infections / Nucleocapsid Proteins / Metal Nanoparticles / Betacoronavirus Type of study: Diagnostic study Language: English Journal: ACS Nano Clinical aspect: Diagnosis Year: 2020
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