SARS-CoV-2 does not replicate in embryonated hen's eggs or in MDCK cell lines.

Barr, Ian G; Rynehart, Cleve; Whitney, Paul; Druce, Julian

Barr, Ian G; Rynehart, Cleve; Whitney, Paul; Druce, Julian.

Barr IG; The WHO Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
Rynehart C; Department of Microbiology and Immunology, The University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
Whitney P; Faculty of Science and Technology, Federation University, Gippsland, VIC, Australia.
Druce J; The WHO Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.

Euro Surveill ; 25(25)2020 06.

Article in English | MEDLINE | ID: covidwho-649992

ABSTRACT

ABSTRACT

The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate embryonated hen's eggs and continuous cell lines that are used to isolate and produce influenza vaccines. We investigated the ability of these substrates to propagate SARS-CoV-2 and found that neither could support SARS-CoV-2 replication.

Subject(s)

Chickens/immunology; Coronavirus/physiology; Influenza Vaccines/immunology; Influenza, Human/prevention & control; Madin Darby Canine Kidney Cells; Receptors, Virus/metabolism; Virus Cultivation/methods; Virus Replication; Animals; Betacoronavirus; COVID-19; Cell Line; Chickens/virology; Coronavirus Infections/epidemiology; Coronavirus Infections/virology; Dogs; Eggs; Humans; Pandemics; Pneumonia, Viral; SARS-CoV-2; Severe Acute Respiratory Syndrome

Keywords

Influenza, SARS-CoV-2, vaccine, respiratory, air-borne infections, viral infections, influenza; severe acute respiratory syndrome SARS, influenza virus, biosafety, vaccines and immunisation, laboratory

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Receptors, Virus / Virus Cultivation / Virus Replication / Influenza Vaccines / Chickens / Coronavirus / Influenza, Human / Madin Darby Canine Kidney Cells Type of study: Observational study / Prognostic study Topics: Vaccines Limits: Animals / Humans Language: English Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: 1560-7917.ES.2020.25.25.2001122

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