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Biotin Functionalized Self-Assembled Peptide Nanofiber as an Adjuvant for Immunomodulatory Response.
Demircan, Muhammed Burak; Tohumeken, Sehmus; Gunduz, Nuray; Khalily, Mohammad Aref; Tekinay, Turgay; Guler, Mustafa O; Tekinay, Ayse B.
  • Demircan MB; Neuroscience Graduate Program, Bilkent University, Ankara, 06800, Turkey.
  • Tohumeken S; Department of Internal Medicine II, Hematology and Oncology, Friedrich-Schiller-University Medical Center, Jena, 07743, Germany.
  • Gunduz N; Leibniz-Institute on Aging, Fritz-Lipmann-Institute, Jena, 07745, Germany.
  • Khalily MA; Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, 06800, Turkey.
  • Tekinay T; Division of Hematologic Malignancies, Johns Hopkins Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD, USA.
  • Guler MO; Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, 06800, Turkey.
  • Tekinay AB; The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Biotechnol J ; 15(12): e2000100, 2020 Dec.
Article in English | MEDLINE | ID: covidwho-650634
ABSTRACT
Biotinylated peptide amphiphile (Biotin-PA) nanofibers, are designed as a noncovalent binding location for antigens, which are adjuvants to enhance, accelerate, and prolong the immune response triggered by antigens. Presenting antigens on synthetic Biotin-PA nanofibers generated a higher immune response than the free antigens delivered with a cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODN) (TLR9 agonist) adjuvant. Antigen attached Biotin-PA nanofibers trigger splenocytes to produce high levels of cytokines (IFN-γ, IL-12, TNF-α, and IL-6) and to exhibit a superior cross-presentation of the antigen. Both Biotin-PA nanofibers and CpG ODN induce a Th-1-biased IgG subclass response; however, delivering the antigen with Biotin-PA nanofibers induce significantly greater production of total IgG and subclasses of IgG compared to delivering the antigen with CpG ODN. Contrary to CpG ODN, Biotin-PA nanofibers also enhance antigen-specific splenocyte proliferation and increase the proportion of the antigen-specific CD8(+) T cells. Given their biodegradability and biocompatibility, Biotin-PA nanofibers have a significant potential in immunoengineering applications as a biomaterial for the delivery of a diverse set of antigens derived from intracellular pathogens, emerging viral diseases such as COVID-19, or cancer cells to induce humoral and cellular immune responses against the antigens.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Peptides / Adjuvants, Immunologic / Nanofibers Type of study: Randomized controlled trials Limits: Animals Language: English Journal: Biotechnol J Journal subject: Biotechnology Year: 2020 Document Type: Article Affiliation country: Biot.202000100

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Peptides / Adjuvants, Immunologic / Nanofibers Type of study: Randomized controlled trials Limits: Animals Language: English Journal: Biotechnol J Journal subject: Biotechnology Year: 2020 Document Type: Article Affiliation country: Biot.202000100